{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Moffitt K"],"funding":["NIAID NIH HHS"],"pagination":["909-16"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4519727"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["22(8)"],"pubmed_abstract":["A pneumococcal whole-cell vaccine (WCV) confers T(H)17-mediated immunogenicity and reduces nasopharyngeal (NP) carriage in mice. Activation of Toll-like receptor 2 (TLR2) has been shown to be important for generating T(H)17 responses, and several lipidated pneumococcal proteins have TLR2-activating properties. Here we investigated the roles of TLR2 and lipidation of proteins in WCV-induced interleukin-17A (IL-17A) responses and protection against NP carriage. Immunization of Tlr2(-/-) mice with WCV conferred significantly lower IL-17A levels and reduced protection against NP carriage, compared to wild-type (WT) mice, suggesting that host TLR2 engagement is required for effective immunity and protection elicited by WCV immunization. Using a WCV with deletion of lgt, the gene encoding the en"],"journal":["Clinical and vaccine immunology : CVI"],"pubmed_title":["T(H)17-Mediated Protection against Pneumococcal Carriage by a Whole-Cell Vaccine Is Dependent on Toll-Like Receptor 2 and Surface Lipoproteins."],"pmcid":["PMC4519727"],"funding_grant_id":["R01 AI100114","K08 AI095352","K08-AI095352","R01-AI100114"],"pubmed_authors":["Howard A","Basset A","Cheung E","Malley R","Moffitt K","Martin S","Herd M"],"additional_accession":[]},"is_claimable":false,"name":"T(H)17-Mediated Protection against Pneumococcal Carriage by a Whole-Cell Vaccine Is Dependent on Toll-Like Receptor 2 and Surface Lipoproteins.","description":"A pneumococcal whole-cell vaccine (WCV) confers T(H)17-mediated immunogenicity and reduces nasopharyngeal (NP) carriage in mice. Activation of Toll-like receptor 2 (TLR2) has been shown to be important for generating T(H)17 responses, and several lipidated pneumococcal proteins have TLR2-activating properties. Here we investigated the roles of TLR2 and lipidation of proteins in WCV-induced interleukin-17A (IL-17A) responses and protection against NP carriage. Immunization of Tlr2(-/-) mice with WCV conferred significantly lower IL-17A levels and reduced protection against NP carriage, compared to wild-type (WT) mice, suggesting that host TLR2 engagement is required for effective immunity and protection elicited by WCV immunization. Using a WCV with deletion of lgt, the gene encoding the en","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Aug","modification":"2025-04-19T07:49:06.965Z","creation":"2019-03-27T01:56:01Z"},"accession":"S-EPMC4519727","cross_references":{"pubmed":["26041040"],"doi":["10.1128/CVI.00118-15","10.1128/cvi.00118-15"]}}