{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Guha Thakurta S"],"funding":["UNL Center for Biotechnology","Nebraska Research Initiative","NCRR NIH HHS","Department of Health and Human Services","Nebraska Department of Health and Human Services"],"pagination":["4798-4810"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4525705"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(11)"],"pubmed_abstract":["Chondrocyte-seeded scaffolds were cultured in an ultrasound (US)-assisted bioreactor, which supplied the cells with acoustic energy around resonance frequencies (~5.0 MHz). Polyurethane-polycarbonate (BM), chitosan (CS) and chitosan-n-butanol (CSB) based scaffolds with varying porosities were chosen and the following US regimen was employed: 15 kPa and 60 kPa, 5 min per application and 6 applications per day for 21 days. Non-stimulated scaffolds served as control. For BM scaffolds, US stimulation significantly impacted cell proliferation and depth-independent cell population density compared to controls. The highest COL2A1/COL1A1 ratios and ACAN mRNA were noted on US-treated BM scaffolds compared to controls. A similar trend was noted on US-treated cell-seeded CS and CSB scaffolds, though "],"journal":["Acta biomaterialia"],"pubmed_title":["Enhanced depth-independent chondrocyte proliferation and phenotype maintenance in an ultrasound bioreactor and an assessment of ultrasound dampening in the scaffold."],"pmcid":["PMC4525705"],"funding_grant_id":["1R21RR024437-01A1","R21 RR024437"],"pubmed_authors":["Kraft M","Viljoen HJ","Guha Thakurta S","Subramanian A"],"additional_accession":[]},"is_claimable":false,"name":"Enhanced depth-independent chondrocyte proliferation and phenotype maintenance in an ultrasound bioreactor and an assessment of ultrasound dampening in the scaffold.","description":"Chondrocyte-seeded scaffolds were cultured in an ultrasound (US)-assisted bioreactor, which supplied the cells with acoustic energy around resonance frequencies (~5.0 MHz). Polyurethane-polycarbonate (BM), chitosan (CS) and chitosan-n-butanol (CSB) based scaffolds with varying porosities were chosen and the following US regimen was employed: 15 kPa and 60 kPa, 5 min per application and 6 applications per day for 21 days. Non-stimulated scaffolds served as control. For BM scaffolds, US stimulation significantly impacted cell proliferation and depth-independent cell population density compared to controls. The highest COL2A1/COL1A1 ratios and ACAN mRNA were noted on US-treated BM scaffolds compared to controls. A similar trend was noted on US-treated cell-seeded CS and CSB scaffolds, though ","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Nov","modification":"2025-05-31T23:33:49.379Z","creation":"2025-05-31T23:33:49.379Z"},"accession":"S-EPMC4525705","cross_references":{"pubmed":["25065549"],"doi":["10.1016/j.actbio.2014.07.013"]}}