biostudies-literatureUnknown6Ciccocioppo R<h4>Introduction</h4>Crohn's disease (CD) is a disabling chronic enteropathy sustained by a harmful T-cell response toward antigens of the gut microbiota in genetically susceptible subjects. Growing evidence highlights the safety and possible efficacy of mesenchymal stem cells (MSCs) as a new therapeutic tool for this condition. Therefore, we aimed to investigate the effects of bone marrow-derived MSCs on pathogenic T cells with a view to clinical application.<h4>Methods</h4>T-cell lines from both inflamed and non-inflamed colonic mucosal specimens of CD patients and from healthy mucosa of control subjects were grown with the antigen muramyl-dipeptide in the absence or presence of donors' MSCs. The MSC effects were evaluated in terms of T-cell viability, apoptotic rate, proliferative respoStem cell research & therapy137https://www.ebi.ac.uk/biostudies/studies/S-EPMC4529692biostudies-literatureEx vivo immunosuppressive effects of mesenchymal stem cells on Crohn's disease mucosal T cells are largely dependent on indoleamine 2,3-dioxygenase activity and cell-cell contact.PMC4529692Monti MCangemi GCKruzliak PMartinetti MCervio MBetti EGurrado AGobbi PBozzi VGallia ABadulli CAlvisi CVisai LPicone CCiccocioppo RPecci ADionigi PBernardo MECorazza GRfalseEx vivo immunosuppressive effects of mesenchymal stem cells on Crohn's disease mucosal T cells are largely dependent on indoleamine 2,3-dioxygenase activity and cell-cell contact.<h4>Introduction</h4>Crohn's disease (CD) is a disabling chronic enteropathy sustained by a harmful T-cell response toward antigens of the gut microbiota in genetically susceptible subjects. Growing evidence highlights the safety and possible efficacy of mesenchymal stem cells (MSCs) as a new therapeutic tool for this condition. Therefore, we aimed to investigate the effects of bone marrow-derived MSCs on pathogenic T cells with a view to clinical application.<h4>Methods</h4>T-cell lines from both inflamed and non-inflamed colonic mucosal specimens of CD patients and from healthy mucosa of control subjects were grown with the antigen muramyl-dipeptide in the absence or presence of donors' MSCs. The MSC effects were evaluated in terms of T-cell viability, apoptotic rate, proliferative respo2015-01-01T00:00:00Z2015 Jul2026-06-14T03:30:03.871Z2019-06-06T14:43:25ZS-EPMC45296922620637610.1186/s13287-015-0122-1