{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["McErlean CM"],"funding":["Cancer Research UK","NHS","EPSRC","National Institute for Health Research (NIHR)","This study benefited from CRUK","Dept of Health","MRC"],"pagination":["96-102"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4542549"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["101"],"pubmed_abstract":["Methods of monitoring drug toxicity in off-target organs are very important in the development of effective and safe drugs. Standard 2-D techniques, such as histology, are prone to sampling errors and can miss important information. We demonstrate a novel application of optical computed tomography (CT) imaging to quantitatively assess, in 3-D, the response of adult murine spleen to off-target drug toxicity induced by treatment with the vascular disrupting agent ZD6126. Reconstructed images from optical CT scans sensitive to haemoglobin absorption reveal detailed, high-contrast 3-D maps of splenic structure and microvasculature. A significant difference in total splenic volume was found between vehicle and ZD6126-treated cohorts, with mean volumes of 61±3mm(3) and 44±3mm(3) respectively (bo"],"journal":["Microvascular research"],"pubmed_title":["Detecting microvascular changes in the mouse spleen using optical computed tomography."],"pmcid":["PMC4542549"],"funding_grant_id":["NF-SI-0512-10162","C1060/A16464","16464","C1060/A10334"],"pubmed_authors":["Collins DJ","Leach MO","Robinson SP","Doran SJ","McErlean CM","Boult JK"],"additional_accession":[]},"is_claimable":false,"name":"Detecting microvascular changes in the mouse spleen using optical computed tomography.","description":"Methods of monitoring drug toxicity in off-target organs are very important in the development of effective and safe drugs. Standard 2-D techniques, such as histology, are prone to sampling errors and can miss important information. We demonstrate a novel application of optical computed tomography (CT) imaging to quantitatively assess, in 3-D, the response of adult murine spleen to off-target drug toxicity induced by treatment with the vascular disrupting agent ZD6126. Reconstructed images from optical CT scans sensitive to haemoglobin absorption reveal detailed, high-contrast 3-D maps of splenic structure and microvasculature. A significant difference in total splenic volume was found between vehicle and ZD6126-treated cohorts, with mean volumes of 61±3mm(3) and 44±3mm(3) respectively (bo","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Sep","modification":"2025-04-19T08:28:06.809Z","creation":"2019-03-27T01:57:04Z"},"accession":"S-EPMC4542549","cross_references":{"pubmed":["26142118"],"doi":["10.1016/j.mvr.2015.06.008"]}}