{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liu Y"],"funding":["National High Technology Research and Development Program of China","National Natural Science Foundation of China (CN)","NIAAA NIH HHS"],"pagination":["121"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4546291"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14"],"pubmed_abstract":["<h4>Background</h4>L-Threonine is an important amino acid for animal feed. Though the industrial fermentation technology of threonine achieved a very high level, there is still significant room to further improve the industrial strains. The biosensor-based high-throughput screening (HTS) technology has demonstrated its powerful applications. Unfortunately, for most of valuable fine chemicals such as threonine, a HTS system has not been established mainly due to the absence of a suitable biosensor. In this study, we developed a HTS method to gain high-yielding threonine-producing strains.<h4>Results</h4>Novel threonine sensing promoters including cysJp and cysHp were discovered by proteomic analyses of Escherichia coli in response to extracellular threonine challenges. The HTS method was co"],"journal":["Microbial cell factories"],"pubmed_title":["Developing a high-throughput screening method for threonine overproduction based on an artificial promoter."],"pmcid":["PMC4546291"],"funding_grant_id":["2012AA023402","31300041","L40 AA023402"],"pubmed_authors":["Liu Y","Zhang D","Zhang T","Zhang Z","Cao G","Sun C","Ma Y","Li Q","Zhou W","Zheng P","Wang X","Sun J"],"additional_accession":[]},"is_claimable":false,"name":"Developing a high-throughput screening method for threonine overproduction based on an artificial promoter.","description":"<h4>Background</h4>L-Threonine is an important amino acid for animal feed. Though the industrial fermentation technology of threonine achieved a very high level, there is still significant room to further improve the industrial strains. The biosensor-based high-throughput screening (HTS) technology has demonstrated its powerful applications. Unfortunately, for most of valuable fine chemicals such as threonine, a HTS system has not been established mainly due to the absence of a suitable biosensor. In this study, we developed a HTS method to gain high-yielding threonine-producing strains.<h4>Results</h4>Novel threonine sensing promoters including cysJp and cysHp were discovered by proteomic analyses of Escherichia coli in response to extracellular threonine challenges. The HTS method was co","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Aug","modification":"2025-04-04T09:22:26.927Z","creation":"2019-03-27T01:57:10Z"},"accession":"S-EPMC4546291","cross_references":{"pubmed":["26296345"],"doi":["10.1186/s12934-015-0311-8"]}}