biostudies-literatureLiu YNational High Technology Research and Development Program of ChinaNational Natural Science Foundation of China (CN)NIAAA NIH HHS121https://www.ebi.ac.uk/biostudies/studies/S-EPMC4546291biostudies-literatureUnknown14<h4>Background</h4>L-Threonine is an important amino acid for animal feed. Though the industrial fermentation technology of threonine achieved a very high level, there is still significant room to further improve the industrial strains. The biosensor-based high-throughput screening (HTS) technology has demonstrated its powerful applications. Unfortunately, for most of valuable fine chemicals such as threonine, a HTS system has not been established mainly due to the absence of a suitable biosensor. In this study, we developed a HTS method to gain high-yielding threonine-producing strains.<h4>Results</h4>Novel threonine sensing promoters including cysJp and cysHp were discovered by proteomic analyses of Escherichia coli in response to extracellular threonine challenges. The HTS method was coMicrobial cell factoriesDeveloping a high-throughput screening method for threonine overproduction based on an artificial promoter.PMC45462912012AA02340231300041L40 AA023402Liu YZhang DZhang TZhang ZCao GSun CMa YLi QZhou WZheng PWang XSun JfalseDeveloping a high-throughput screening method for threonine overproduction based on an artificial promoter.<h4>Background</h4>L-Threonine is an important amino acid for animal feed. Though the industrial fermentation technology of threonine achieved a very high level, there is still significant room to further improve the industrial strains. The biosensor-based high-throughput screening (HTS) technology has demonstrated its powerful applications. Unfortunately, for most of valuable fine chemicals such as threonine, a HTS system has not been established mainly due to the absence of a suitable biosensor. In this study, we developed a HTS method to gain high-yielding threonine-producing strains.<h4>Results</h4>Novel threonine sensing promoters including cysJp and cysHp were discovered by proteomic analyses of Escherichia coli in response to extracellular threonine challenges. The HTS method was co2015-01-01T00:00:00Z2015 Aug2025-04-04T09:22:26.927Z2019-03-27T01:57:10ZS-EPMC45462912629634510.1186/s12934-015-0311-8