{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(8)"],"submitter":["Miyai M"],"pubmed_abstract":["Comprehensive immunological evaluation is crucial for monitoring patients undergoing antigen-specific cancer immunotherapy. The identification and quantification of T cell responses is most important for the further development of such therapies. Using well-characterized clinical samples from a high responder patient (TK-f01) in an NY-ESO-1f peptide vaccine study, we performed high-throughput T cell receptor β-chain (TCRB) gene next generation sequencing (NGS) to monitor the frequency of NY-ESO-1-specific CD8+ T cells. We compared these results with those of conventional immunological assays, such as IFN-γ capture, tetramer binding and limiting dilution clonality assays. We sequenced human TCRB complementarity-determining region 3 (CDR3) rearrangements of two NY-ESO-1f-specific CD8+ T cell"],"journal":["PloS one"],"pagination":["e0136086"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4546392"],"repository":["biostudies-literature"],"pubmed_title":["Detection and Tracking of NY-ESO-1-Specific CD8+ T Cells by High-Throughput T Cell Receptor β (TCRB) Gene Rearrangements Sequencing in a Peptide-Vaccinated Patient."],"pmcid":["PMC4546392"],"pubmed_authors":["Matsushita H","Miyai M","Eikawa S","Nakajima J","Hosoi A","Nakayama E","Iino T","Isobe M","Uenaka A","Kakimi K","Udono H"],"additional_accession":[]},"is_claimable":false,"name":"Detection and Tracking of NY-ESO-1-Specific CD8+ T Cells by High-Throughput T Cell Receptor β (TCRB) Gene Rearrangements Sequencing in a Peptide-Vaccinated Patient.","description":"Comprehensive immunological evaluation is crucial for monitoring patients undergoing antigen-specific cancer immunotherapy. The identification and quantification of T cell responses is most important for the further development of such therapies. Using well-characterized clinical samples from a high responder patient (TK-f01) in an NY-ESO-1f peptide vaccine study, we performed high-throughput T cell receptor β-chain (TCRB) gene next generation sequencing (NGS) to monitor the frequency of NY-ESO-1-specific CD8+ T cells. We compared these results with those of conventional immunological assays, such as IFN-γ capture, tetramer binding and limiting dilution clonality assays. We sequenced human TCRB complementarity-determining region 3 (CDR3) rearrangements of two NY-ESO-1f-specific CD8+ T cell","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015","modification":"2025-04-04T12:39:25.057Z","creation":"2019-03-26T23:35:27Z"},"accession":"S-EPMC4546392","cross_references":{"pubmed":["26291626"],"doi":["10.1371/journal.pone.0136086"]}}