biostudies-literature00420Jones DMWellcome Trust13449https://www.ebi.ac.uk/biostudies/studies/S-EPMC4547133biostudies-literatureUnknown5We have produced a novel, simple and rapid method utilising genetically encodable FRET-based biosensors to permit the detection of HIV-1 virion fusion in living cells. These biosensors show high sensitivity both spatially and temporally, and allow the real-time recovery of HIV-1 fusion kinetics in both single cells and cell populations simultaneously.Scientific reportsImaging real-time HIV-1 virion fusion with FRET-based biosensors.PMC4547133090532/Z/09/ZJones DMPadilla-Parra S42falseImaging real-time HIV-1 virion fusion with FRET-based biosensors.We have produced a novel, simple and rapid method utilising genetically encodable FRET-based biosensors to permit the detection of HIV-1 virion fusion in living cells. These biosensors show high sensitivity both spatially and temporally, and allow the real-time recovery of HIV-1 fusion kinetics in both single cells and cell populations simultaneously.2015-01-01T00:00:00Z2015 Aug2024-12-04T11:44:27.529Z2019-03-27T01:57:12ZS-EPMC45471332630021210.1038/srep13449