{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Skowera A"],"funding":["National Institute for Health Research (NIHR)","Juvenile Diabetes Research Foundation","Wellcome Trust","Juvenile Diabetes Research Foundation (JDRF) Autoimmunity Centers Consortium"],"pagination":["916-925"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4557541"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["64(3)"],"pubmed_abstract":["Autoreactive CD8 T cells play a central role in the destruction of pancreatic islet β-cells that leads to type 1 diabetes, yet the key features of this immune-mediated process remain poorly defined. In this study, we combined high-definition polychromatic flow cytometry with ultrasensitive peptide-human leukocyte antigen class I tetramer staining to quantify and characterize β-cell-specific CD8 T cell populations in patients with recent-onset type 1 diabetes and healthy control subjects. Remarkably, we found that β-cell-specific CD8 T cell frequencies in peripheral blood were similar between subject groups. In contrast to healthy control subjects, however, patients with newly diagnosed type 1 diabetes displayed hallmarks of antigen-driven expansion uniquely within the β-cell-specific CD8 T"],"journal":["Diabetes"],"pubmed_title":["β-cell-specific CD8 T cell phenotype in type 1 diabetes reflects chronic autoantigen exposure."],"pmcid":["PMC4557541"],"funding_grant_id":["100326/Z/12/Z","type 1 diabetes 217194","17-2012-352","1-2007-1803","100326","100327"],"pubmed_authors":["Heck S","Skowera A","Sewell AK","McLaren JE","Price DA","Eichmann M","Bingley PJ","Dayan CM","Peakman M","Matthews KK","Kronenberg-Versteeg D","Powrie J","Miles JJ","Dolton G","Ladell K","Knight RR","Gostick E"],"additional_accession":[]},"is_claimable":false,"name":"β-cell-specific CD8 T cell phenotype in type 1 diabetes reflects chronic autoantigen exposure.","description":"Autoreactive CD8 T cells play a central role in the destruction of pancreatic islet β-cells that leads to type 1 diabetes, yet the key features of this immune-mediated process remain poorly defined. In this study, we combined high-definition polychromatic flow cytometry with ultrasensitive peptide-human leukocyte antigen class I tetramer staining to quantify and characterize β-cell-specific CD8 T cell populations in patients with recent-onset type 1 diabetes and healthy control subjects. Remarkably, we found that β-cell-specific CD8 T cell frequencies in peripheral blood were similar between subject groups. In contrast to healthy control subjects, however, patients with newly diagnosed type 1 diabetes displayed hallmarks of antigen-driven expansion uniquely within the β-cell-specific CD8 T","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Mar","modification":"2025-04-26T04:04:54.63Z","creation":"2019-03-27T01:57:42Z"},"accession":"S-EPMC4557541","cross_references":{"pubmed":["25249579"],"doi":["10.2337/db14-0332"]}}