{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Geno KA"],"funding":["NIAID NIH HHS","NHLBI NIH HHS","NIH"],"pagination":["106-10"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4560653"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["424"],"pubmed_abstract":["The human opsonin ficolin-2 (L-ficolin) is an innate pattern-recognizing molecule that binds to acetylated moieties. Upon binding, ficolin-2 activates complement through the lectin pathway, opsonizing the target to promote phagocytic clearance. Ficolin-2 has been found to interact with a growing number of pathogenic bacteria, fungi, and viruses. Ficolin-2 also has proposed roles in host homeostasis, including the clearance of apoptotic cells. Consequently, there is an increased interest in studying ficolin-2, and access to purified ficolin-2 is necessary for these studies. Ficolin-2 purified from serum, plasma, or cell culture supernatants has been a useful tool in the characterization of ficolin-2 function; however, available protocols are laborious and inefficient, requiring additional p"],"journal":["Journal of immunological methods"],"pubmed_title":["Rapid and efficient purification of ficolin-2 using a disposable CELLine bioreactor."],"pmcid":["PMC4560653"],"funding_grant_id":["T32 HL105346","R56 AI031473"],"pubmed_authors":["Geno KA","Nahm MH","Spencer BL"],"additional_accession":[]},"is_claimable":false,"name":"Rapid and efficient purification of ficolin-2 using a disposable CELLine bioreactor.","description":"The human opsonin ficolin-2 (L-ficolin) is an innate pattern-recognizing molecule that binds to acetylated moieties. Upon binding, ficolin-2 activates complement through the lectin pathway, opsonizing the target to promote phagocytic clearance. Ficolin-2 has been found to interact with a growing number of pathogenic bacteria, fungi, and viruses. Ficolin-2 also has proposed roles in host homeostasis, including the clearance of apoptotic cells. Consequently, there is an increased interest in studying ficolin-2, and access to purified ficolin-2 is necessary for these studies. Ficolin-2 purified from serum, plasma, or cell culture supernatants has been a useful tool in the characterization of ficolin-2 function; however, available protocols are laborious and inefficient, requiring additional p","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Sep","modification":"2025-05-29T20:59:42.09Z","creation":"2019-03-27T01:57:53Z"},"accession":"S-EPMC4560653","cross_references":{"pubmed":["26021447"],"doi":["10.1016/j.jim.2015.05.008"]}}