biostudies-literatureGeno KANIAID NIH HHSNHLBI NIH HHSNIH106-10https://www.ebi.ac.uk/biostudies/studies/S-EPMC4560653biostudies-literatureUnknown424The human opsonin ficolin-2 (L-ficolin) is an innate pattern-recognizing molecule that binds to acetylated moieties. Upon binding, ficolin-2 activates complement through the lectin pathway, opsonizing the target to promote phagocytic clearance. Ficolin-2 has been found to interact with a growing number of pathogenic bacteria, fungi, and viruses. Ficolin-2 also has proposed roles in host homeostasis, including the clearance of apoptotic cells. Consequently, there is an increased interest in studying ficolin-2, and access to purified ficolin-2 is necessary for these studies. Ficolin-2 purified from serum, plasma, or cell culture supernatants has been a useful tool in the characterization of ficolin-2 function; however, available protocols are laborious and inefficient, requiring additional pJournal of immunological methodsRapid and efficient purification of ficolin-2 using a disposable CELLine bioreactor.PMC4560653T32 HL105346R56 AI031473Geno KANahm MHSpencer BLfalseRapid and efficient purification of ficolin-2 using a disposable CELLine bioreactor.The human opsonin ficolin-2 (L-ficolin) is an innate pattern-recognizing molecule that binds to acetylated moieties. Upon binding, ficolin-2 activates complement through the lectin pathway, opsonizing the target to promote phagocytic clearance. Ficolin-2 has been found to interact with a growing number of pathogenic bacteria, fungi, and viruses. Ficolin-2 also has proposed roles in host homeostasis, including the clearance of apoptotic cells. Consequently, there is an increased interest in studying ficolin-2, and access to purified ficolin-2 is necessary for these studies. Ficolin-2 purified from serum, plasma, or cell culture supernatants has been a useful tool in the characterization of ficolin-2 function; however, available protocols are laborious and inefficient, requiring additional p2015-01-01T00:00:00Z2015 Sep2025-05-29T20:59:42.09Z2019-03-27T01:57:53ZS-EPMC45606532602144710.1016/j.jim.2015.05.008