{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yang S"],"funding":["China Agriculture Research System","Basic Research Project"],"pagination":["83"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4570608"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15"],"pubmed_abstract":["Background
Immunomagnetic nanobead (IMNB) labeled with specific antibody, has been demonstrated to be useful for the capturing and detection of viruses.Results
In this study, we developed an imunomagnetic bead based on carboxyl-magnetic beads (MNB) labeled with a single-domain antibody (sdAb) for capturing foot-and-mouth disease (FMD) Asia 1 virus. After magnetic separation, complexes of MNB-sdAb-virus were detected with either a sandwich ELISA or QDs-C5 probe under a fluorescence microscope, and the complexes were used as templates for extraction of total RNA for amplification of the VP1 or 3D gene fragments using RT-PCR and real-time RT-PCR. The Asia 1 VLPs were efficiently captured through IMNB with a high binding rate of 5.09 μg of antigen/μl of bead suspension. Moreove"],"journal":["BMC biotechnology"],"pubmed_title":["Specific detection of foot-and-mouth disease serotype Asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody."],"pmcid":["PMC4570608"],"funding_grant_id":["CARS-39","no.2013FY113300"],"pubmed_authors":["Liu X","Guo J","He J","Yin S","Wang D","Cai J","Shang Y","Yang S","Ma W"],"additional_accession":[]},"is_claimable":false,"name":"Specific detection of foot-and-mouth disease serotype Asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody.","description":"Background
Immunomagnetic nanobead (IMNB) labeled with specific antibody, has been demonstrated to be useful for the capturing and detection of viruses.Results
In this study, we developed an imunomagnetic bead based on carboxyl-magnetic beads (MNB) labeled with a single-domain antibody (sdAb) for capturing foot-and-mouth disease (FMD) Asia 1 virus. After magnetic separation, complexes of MNB-sdAb-virus were detected with either a sandwich ELISA or QDs-C5 probe under a fluorescence microscope, and the complexes were used as templates for extraction of total RNA for amplification of the VP1 or 3D gene fragments using RT-PCR and real-time RT-PCR. The Asia 1 VLPs were efficiently captured through IMNB with a high binding rate of 5.09 μg of antigen/μl of bead suspension. Moreove","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Sep","modification":"2026-06-17T03:17:17.994Z","creation":"2019-03-27T01:58:25Z"},"accession":"S-EPMC4570608","cross_references":{"pubmed":["26369792"],"doi":["10.1186/s12896-015-0201-5"]}}