biostudies-literatureUnknown26(15)Perpelescu MThe CENP-A-specific chaperone HJURP mediates CENP-A deposition at centromeres. The N-terminal region of HJURP is responsible for binding to soluble CENP-A. However, it is unclear whether other regions of HJURP have additional functions for centromere formation and maintenance. In this study, we generated chicken DT40 knockout cell lines and gene replacement constructs for HJURP to assess the additional functions of HJURP in vivo. Our analysis revealed that the middle region of HJURP associates with the Mis18 complex protein M18BP1/KNL2 and that the HJURP-M18BP1 association is required for HJURP function. In addition, on the basis of the analysis of artificial centromeres induced by ectopic HJURP localization, we demonstrate that HJURP exhibits a centromere expansion activity that is separaMolecular biology of the cell2742-54https://www.ebi.ac.uk/biostudies/studies/S-EPMC4571335biostudies-literatureHJURP is involved in the expansion of centromeric chromatin.PMC4571335Perpelescu MFujiyama AIkeo KMisu SToyoda AMonma NObuse CFukagawa THori TfalseHJURP is involved in the expansion of centromeric chromatin.The CENP-A-specific chaperone HJURP mediates CENP-A deposition at centromeres. The N-terminal region of HJURP is responsible for binding to soluble CENP-A. However, it is unclear whether other regions of HJURP have additional functions for centromere formation and maintenance. In this study, we generated chicken DT40 knockout cell lines and gene replacement constructs for HJURP to assess the additional functions of HJURP in vivo. Our analysis revealed that the middle region of HJURP associates with the Mis18 complex protein M18BP1/KNL2 and that the HJURP-M18BP1 association is required for HJURP function. In addition, on the basis of the analysis of artificial centromeres induced by ectopic HJURP localization, we demonstrate that HJURP exhibits a centromere expansion activity that is separa2015-01-01T00:00:00Z2015 Aug2026-04-18T07:38:17.307Z2019-03-27T01:58:27ZS-EPMC45713352606372910.1091/mbc.E15-02-0094