biostudies-literaturePlanas RUZHSwiss National Science FoundationDFG Clinical Research GroupUniversity of ZurichGerman Ministry for Education and Research875-93https://www.ebi.ac.uk/biostudies/studies/S-EPMC4574806biostudies-literatureUnknown2(9)<h4>Objective</h4>Multiple sclerosis (MS) is a disease of the central nervous system with marked heterogeneity in several aspects including pathological processes. Based on infiltrating immune cells, deposition of humoral factors and loss of oligodendrocytes and/or myelin proteins, four lesion patterns have been described. Pattern II is characterized by antibody and complement deposition in addition to T-cell infiltration. MS is considered a T-cell-mediated disease, but until now the study of pathogenic T cells has encountered major challenges, most importantly the limited access of brain-infiltrating T cells. Our objective was to identify, isolate, and characterize brain-infiltrating clonally expanded T cells in pattern II MS lesions.<h4>Methods</h4>We used next-generation sequencing to iAnnals of clinical and translational neurologyCentral role of Th2/Tc2 lymphocytes in pattern II multiple sclerosis lesions.PMC4574806KFO 228/1146945SNF: 310030_146945FK-13-046310030SFB 841Heesen COrtiz YSalinas-Riester GBruck WSospedra MJelcic IVilarrasa NPlanas RMetz IMartin RfalseCentral role of Th2/Tc2 lymphocytes in pattern II multiple sclerosis lesions.<h4>Objective</h4>Multiple sclerosis (MS) is a disease of the central nervous system with marked heterogeneity in several aspects including pathological processes. Based on infiltrating immune cells, deposition of humoral factors and loss of oligodendrocytes and/or myelin proteins, four lesion patterns have been described. Pattern II is characterized by antibody and complement deposition in addition to T-cell infiltration. MS is considered a T-cell-mediated disease, but until now the study of pathogenic T cells has encountered major challenges, most importantly the limited access of brain-infiltrating T cells. Our objective was to identify, isolate, and characterize brain-infiltrating clonally expanded T cells in pattern II MS lesions.<h4>Methods</h4>We used next-generation sequencing to i2015-01-01T00:00:00Z2015 Sep2026-05-03T06:55:01.465Z2019-03-27T01:58:38ZS-EPMC45748062640151010.1002/acn3.218