{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["de S e Silva JM"],"funding":["European Research Council"],"pagination":["14088"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4585898"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["5"],"pubmed_abstract":["Low inherent contrast in soft tissues has been limiting the use of X-ray absorption micro-computed tomography (micro-CT) to access high-resolution structural information of animal organs. The staining agents used in micro-CT to improve the contrast fail in providing high-quality images of whole organs of animals due to diffusion problems of the staining agent into the sample. We demonstrate a staining protocol that incorporates a biochemical conditioning step prior to exposure to the staining agent that succeeds in overcoming the diffusion problems, thus quickly providing high-quality micro-CT images of whole organs of mammals. Besides of yielding non-distorted three-dimensional information at the same spatial resolution accessible in histological sections, micro-CT images of whole organs "],"journal":["Scientific reports"],"pubmed_title":["Three-dimensional non-destructive soft-tissue visualization with X-ray staining micro-tomography."],"pmcid":["PMC4585898"],"funding_grant_id":["240142"],"pubmed_authors":["Zanette I","Kimm MA","Cardoso MB","Noel PB","Pfeiffer F","de S e Silva JM"],"additional_accession":[]},"is_claimable":false,"name":"Three-dimensional non-destructive soft-tissue visualization with X-ray staining micro-tomography.","description":"Low inherent contrast in soft tissues has been limiting the use of X-ray absorption micro-computed tomography (micro-CT) to access high-resolution structural information of animal organs. The staining agents used in micro-CT to improve the contrast fail in providing high-quality images of whole organs of animals due to diffusion problems of the staining agent into the sample. We demonstrate a staining protocol that incorporates a biochemical conditioning step prior to exposure to the staining agent that succeeds in overcoming the diffusion problems, thus quickly providing high-quality micro-CT images of whole organs of mammals. Besides of yielding non-distorted three-dimensional information at the same spatial resolution accessible in histological sections, micro-CT images of whole organs ","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Sep","modification":"2025-04-21T20:24:39.282Z","creation":"2019-03-27T01:59:07Z"},"accession":"S-EPMC4585898","cross_references":{"pubmed":["26404036"],"doi":["10.1038/srep14088"]}}