{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Du H"],"funding":["Intramural NIH HHS","NIGMS NIH HHS"],"pagination":["641-9"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4589277"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["31(5)"],"pubmed_abstract":["Maintenance of genomic integrity during antigen receptor gene rearrangements requires (1) regulated access of the V(D)J recombinase to specific loci and (2) generation of double-strand DNA breaks only after recognition of a pair of matched recombination signal sequences (RSSs). Here we recapitulate both key aspects of regulated recombinase accessibility in a cell-free system using plasmid substrates assembled into chromatin. We show that recruitment of the SWI/SNF chromatin-remodeling complex to both RSSs increases coupled cleavage by RAG1 and RAG2 proteins. SWI/SNF functions by altering local chromatin structure in the absence of RNA polymerase II-dependent transcription or histone modifications. These observations demonstrate a direct role for cis-sequence-regulated local chromatin remod"],"journal":["Molecular cell"],"pubmed_title":["Activation of 12/23-RSS-dependent RAG cleavage by hSWI/SNF complex in the absence of transcription."],"pmcid":["PMC4589277"],"funding_grant_id":["R01 GM038925","ZIA AG000374-03","GM38925","Z01 AG000524-03"],"pubmed_authors":["Du H","Ishii H","Pazin MJ","Sen R"],"additional_accession":[]},"is_claimable":false,"name":"Activation of 12/23-RSS-dependent RAG cleavage by hSWI/SNF complex in the absence of transcription.","description":"Maintenance of genomic integrity during antigen receptor gene rearrangements requires (1) regulated access of the V(D)J recombinase to specific loci and (2) generation of double-strand DNA breaks only after recognition of a pair of matched recombination signal sequences (RSSs). Here we recapitulate both key aspects of regulated recombinase accessibility in a cell-free system using plasmid substrates assembled into chromatin. We show that recruitment of the SWI/SNF chromatin-remodeling complex to both RSSs increases coupled cleavage by RAG1 and RAG2 proteins. SWI/SNF functions by altering local chromatin structure in the absence of RNA polymerase II-dependent transcription or histone modifications. These observations demonstrate a direct role for cis-sequence-regulated local chromatin remod","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 Sep","modification":"2025-06-01T12:01:39.208Z","creation":"2025-06-01T12:01:39.208Z"},"accession":"S-EPMC4589277","cross_references":{"pubmed":["18775324"],"doi":["10.1016/j.molcel.2008.08.012"]}}