biostudies-literatureDeRosa BANIMH NIH HHSNINDS NIH HHSNIHHussman Foundation244-57https://www.ebi.ac.uk/biostudies/studies/S-EPMC4593758biostudies-literatureUnknown68<h4>Background</h4>GABAergic synaptic transmission is known to play a critical role in the assembly of neuronal circuits during development and is responsible for maintaining the balance between excitatory and inhibitory signaling in the brain during maturation into adulthood. Importantly, defects in GABAergic neuronal function and signaling have been linked to a number of neurological diseases, including autism spectrum disorders, schizophrenia, and epilepsy. With patient-specific induced pluripotent stem cell (iPSC)-based models of neurological disease, it is now possible to investigate the disease mechanisms that underlie deficits in GABAergic function in affected human neurons. To that end, tools that enable the labeling and purification of viable GABAergic neurons from human pluripoteMolecular and cellular neuroscienceshVGAT-mCherry: A novel molecular tool for analysis of GABAergic neurons derived from human pluripotent stem cells.PMC4593758P50NS071674P50 NS071674R01 MH080647Cukier HNVance JMBelle KCDykxhoorn DMThomas BJDeRosa BAPericak-Vance MAfalsehVGAT-mCherry: A novel molecular tool for analysis of GABAergic neurons derived from human pluripotent stem cells.<h4>Background</h4>GABAergic synaptic transmission is known to play a critical role in the assembly of neuronal circuits during development and is responsible for maintaining the balance between excitatory and inhibitory signaling in the brain during maturation into adulthood. Importantly, defects in GABAergic neuronal function and signaling have been linked to a number of neurological diseases, including autism spectrum disorders, schizophrenia, and epilepsy. With patient-specific induced pluripotent stem cell (iPSC)-based models of neurological disease, it is now possible to investigate the disease mechanisms that underlie deficits in GABAergic function in affected human neurons. To that end, tools that enable the labeling and purification of viable GABAergic neurons from human pluripote2015-01-01T00:00:00Z2015 Sep2025-04-04T13:25:08.656Z2019-03-27T01:59:33ZS-EPMC45937582628497910.1016/j.mcn.2015.08.007