biostudies-literatureUnknown6Omer HBacillus cereus is a Gram-positive pathogen that causes a wide variety of diseases in humans. It secretes into the extracellular milieu proteins that may contribute directly or indirectly to its virulence. EntD is a novel exoprotein identified by proteogenomics of B. cereus ATCC 14579. We constructed a ΔentD mutant and analyzed the impact of entD disruption on the cellular proteome and exoproteome isolated from early, late, and stationary-phase cultures. We identified 308 and 79 proteins regulated by EntD in the cellular proteome and the exoproteome, respectively. The contribution of these proteins to important virulence-associated functions, including central metabolism, cell structure, antioxidative ability, cell motility, and toxin production, are presented. The proteomic data were corrFrontiers in microbiology1004https://www.ebi.ac.uk/biostudies/studies/S-EPMC4595770biostudies-literatureProteomics identifies Bacillus cereus EntD as a pivotal protein for the production of numerous virulence factors.PMC4595770Alpha-Bazin BArmengaud JBrunet JLDuport COmer HfalseProteomics identifies Bacillus cereus EntD as a pivotal protein for the production of numerous virulence factors.Bacillus cereus is a Gram-positive pathogen that causes a wide variety of diseases in humans. It secretes into the extracellular milieu proteins that may contribute directly or indirectly to its virulence. EntD is a novel exoprotein identified by proteogenomics of B. cereus ATCC 14579. We constructed a ΔentD mutant and analyzed the impact of entD disruption on the cellular proteome and exoproteome isolated from early, late, and stationary-phase cultures. We identified 308 and 79 proteins regulated by EntD in the cellular proteome and the exoproteome, respectively. The contribution of these proteins to important virulence-associated functions, including central metabolism, cell structure, antioxidative ability, cell motility, and toxin production, are presented. The proteomic data were corr2015-01-01T00:00:00Z20152026-04-08T02:01:55.836Z2019-03-27T01:59:38ZS-EPMC45957702650061010.3389/fmicb.2015.01004