{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["5"],"submitter":["Kern F"],"pubmed_abstract":["Since their discovery in the soil bacterium Sorangium cellulosum, epothilones have emerged as a valuable substance class with promising anti-tumor activity. Because of their benefits in the treatment of cancer and neurodegenerative diseases, epothilones are targets for drug design and pharmaceutical research. The final step of their biosynthesis - a cytochrome P450 mediated epoxidation of epothilone C/D to A/B by CYP167A1 (EpoK) - needs significant improvement, in particular regarding the efficiency of its redox partners. Therefore, we have investigated the ability of various hetero- and homologous redox partners to transfer electrons to EpoK. Hereby, a new hybrid system was established with conversion rates eleven times higher and Vmax of more than seven orders of magnitudes higher as com"],"journal":["Scientific reports"],"pagination":["14881"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4597204"],"repository":["biostudies-literature"],"pubmed_title":["Highly Efficient CYP167A1 (EpoK) dependent Epothilone B Formation and Production of 7-Ketone Epothilone D as a New Epothilone Derivative."],"pmcid":["PMC4597204"],"pubmed_authors":["Volmer DA","Bernhardt R","Jacquot JP","Ewen KM","Kern F","Dier TK","Khatri Y"],"additional_accession":[]},"is_claimable":false,"name":"Highly Efficient CYP167A1 (EpoK) dependent Epothilone B Formation and Production of 7-Ketone Epothilone D as a New Epothilone Derivative.","description":"Since their discovery in the soil bacterium Sorangium cellulosum, epothilones have emerged as a valuable substance class with promising anti-tumor activity. Because of their benefits in the treatment of cancer and neurodegenerative diseases, epothilones are targets for drug design and pharmaceutical research. The final step of their biosynthesis - a cytochrome P450 mediated epoxidation of epothilone C/D to A/B by CYP167A1 (EpoK) - needs significant improvement, in particular regarding the efficiency of its redox partners. Therefore, we have investigated the ability of various hetero- and homologous redox partners to transfer electrons to EpoK. Hereby, a new hybrid system was established with conversion rates eleven times higher and Vmax of more than seven orders of magnitudes higher as com","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Oct","modification":"2025-04-04T10:50:08.547Z","creation":"2019-03-27T01:59:42Z"},"accession":"S-EPMC4597204","cross_references":{"pubmed":["26445909"],"doi":["10.1038/srep14881"]}}