biostudies-literatureUnknown5Coleman WLBloomsburg University Research and ScholarshipUndergraduate Research, Scholarship, and Creative ActivitiesProteins known to function during cell-cell communication and exocytosis in neurons and other secretory cells have recently been reported in human sperm. Synapsins are a group of proteins that have been very well characterized in neurons, but little is known about synapsin function in other cell types. Based upon previous findings and the known function of synapsin, we tested the hypothesis that synapsin I was present in human sperm. Washed, capacitated, and acrosome induced sperm preparations were used to evaluate the functional distribution of synapsin I using immunocytochemistry. Protein extracts from mouse brain, mouse testis/epididymis, and human semen were used for protein blotting techniques. Immunolocalization revealed synapsin I was enriched in the sperm equatorial segment. ProteiFEBS open bio801-8https://www.ebi.ac.uk/biostudies/studies/S-EPMC4600850biostudies-literatureFunctional distribution of synapsin I in human sperm.PMC4600850Coleman WLVenditti JJKulp ACfalseFunctional distribution of synapsin I in human sperm.Proteins known to function during cell-cell communication and exocytosis in neurons and other secretory cells have recently been reported in human sperm. Synapsins are a group of proteins that have been very well characterized in neurons, but little is known about synapsin function in other cell types. Based upon previous findings and the known function of synapsin, we tested the hypothesis that synapsin I was present in human sperm. Washed, capacitated, and acrosome induced sperm preparations were used to evaluate the functional distribution of synapsin I using immunocytochemistry. Protein extracts from mouse brain, mouse testis/epididymis, and human semen were used for protein blotting techniques. Immunolocalization revealed synapsin I was enriched in the sperm equatorial segment. Protei2015-01-01T00:00:00Z20152025-04-04T20:15:57.738Z2019-03-27T01:59:52ZS-EPMC46008502656647410.1016/j.fob.2015.09.006