{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Georis I"],"funding":["NIGMS NIH HHS"],"pagination":["824-37"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4615157"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(8)"],"pubmed_abstract":["The first step in executing the genetic program of a cell is production of mRNA. In yeast, almost every gene is transcribed as multiple distinct isoforms, differing at their 5' and/or 3' termini. However, the implications and functional significance of the transcriptome-wide diversity of mRNA termini remains largely unexplored. In this paper, we show that the GAT1 gene, encoding a transcriptional activator of nitrogen-responsive catabolic genes, produces a variety of mRNAs differing in their 5' and 3' termini. Alternative transcription initiation leads to the constitutive, low level production of 2 full length proteins differing in their N-termini, whereas premature transcriptional termination generates a short, highly nitrogen catabolite repression- (NCR-) sensitive transcript that, as far as we can determine, is not translated under the growth conditions we used, but rather likely protects the cell from excess Gat1."],"journal":["RNA biology"],"pubmed_title":["Premature termination of GAT1 transcription explains paradoxical negative correlation between nitrogen-responsive mRNA, but constitutive low-level protein production."],"pmcid":["PMC4615157"],"funding_grant_id":["R01 GM035642","GM-35642"],"pubmed_authors":["Tate JJ","Isabelle G","Vierendeels F","Cooper TG","Dubois E","Georis I"],"additional_accession":[]},"is_claimable":false,"name":"Premature termination of GAT1 transcription explains paradoxical negative correlation between nitrogen-responsive mRNA, but constitutive low-level protein production.","description":"The first step in executing the genetic program of a cell is production of mRNA. In yeast, almost every gene is transcribed as multiple distinct isoforms, differing at their 5' and/or 3' termini. However, the implications and functional significance of the transcriptome-wide diversity of mRNA termini remains largely unexplored. In this paper, we show that the GAT1 gene, encoding a transcriptional activator of nitrogen-responsive catabolic genes, produces a variety of mRNAs differing in their 5' and 3' termini. Alternative transcription initiation leads to the constitutive, low level production of 2 full length proteins differing in their N-termini, whereas premature transcriptional termination generates a short, highly nitrogen catabolite repression- (NCR-) sensitive transcript that, as far as we can determine, is not translated under the growth conditions we used, but rather likely protects the cell from excess Gat1.","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015","modification":"2021-02-20T14:39:16Z","creation":"2019-03-27T02:00:32Z"},"accession":"S-EPMC4615157","cross_references":{"pubmed":["26259534"],"doi":["10.1080/15476286.2015.1058476"]}}