biostudies-literatureBohr TNIGMS NIH HHSNIH HHS233-42https://www.ebi.ac.uk/biostudies/studies/S-EPMC4621841biostudies-literatureUnknown211(2)Homologue synapsis is required for meiotic chromosome segregation, but how synapsis is initiated between chromosomes is poorly understood. In Caenorhabditis elegans, synapsis and a checkpoint that monitors synapsis depend on pairing centers (PCs), cis-acting loci that interact with nuclear envelope proteins, such as SUN-1, to access cytoplasmic microtubules. Here, we report that spindle assembly checkpoint (SAC) components MAD-1, MAD-2, and BUB-3 are required to negatively regulate synapsis and promote the synapsis checkpoint response. Both of these roles are independent of a conserved component of the anaphase-promoting complex, indicating a unique role for these proteins in meiotic prophase. MAD-1 and MAD-2 localize to the periphery of meiotic nuclei and interact with SUN-1, suggesting aThe Journal of cell biologySpindle assembly checkpoint proteins regulate and monitor meiotic synapsis in C. elegans.PMC4621841R01GM097144T32 GM008646R01 GM097144P40 OD010440T32GM008646R25 GM058903Bhalla NBohr TKlee ENelson CRfalseSpindle assembly checkpoint proteins regulate and monitor meiotic synapsis in C. elegans.Homologue synapsis is required for meiotic chromosome segregation, but how synapsis is initiated between chromosomes is poorly understood. In Caenorhabditis elegans, synapsis and a checkpoint that monitors synapsis depend on pairing centers (PCs), cis-acting loci that interact with nuclear envelope proteins, such as SUN-1, to access cytoplasmic microtubules. Here, we report that spindle assembly checkpoint (SAC) components MAD-1, MAD-2, and BUB-3 are required to negatively regulate synapsis and promote the synapsis checkpoint response. Both of these roles are independent of a conserved component of the anaphase-promoting complex, indicating a unique role for these proteins in meiotic prophase. MAD-1 and MAD-2 localize to the periphery of meiotic nuclei and interact with SUN-1, suggesting a2015-01-01T00:00:00Z2015 Oct2025-04-06T19:45:20.461Z2019-03-27T02:00:49ZS-EPMC46218412648355510.1083/jcb.201409035