biostudies-literatureCortina-Ceballos BCancer Research UK516-24https://www.ebi.ac.uk/biostudies/studies/S-EPMC4622655biostudies-literatureUnknown7(3)The B cell antigen receptor repertoire is highly diverse and constantly modified by clonal selection. High-throughput DNA sequencing (HTS) of the lymphocyte repertoire (Rep-Seq) represents a promising technology to explore such diversity ex-vivo and assist in the identification of antigen-specific antibodies based on molecular signatures of clonal selection. Therefore, integrative tools for repertoire reconstruction and analysis from antibody sequences are needed. We developed ImmunediveRity, a stand-alone pipeline primarily based in R programming for the integral analysis of B cell repertoire data generated by HTS. The pipeline integrates GNU software and in house scripts to perform quality filtering, sequencing noise correction and repertoire reconstruction based on V, D and J segment asmAbsReconstructing and mining the B cell repertoire with ImmunediveRsity.PMC462265514356Godoy-Lozano EERomero GMartinez-Barnetche JCortina-Ceballos BVelasco-Herrera Mdel CVelazquez-Ramirez DVargas-Chavez CAguilar-Salgado AMoreno JSamano-Sanchez HTellez-Sosa JfalseReconstructing and mining the B cell repertoire with ImmunediveRsity.The B cell antigen receptor repertoire is highly diverse and constantly modified by clonal selection. High-throughput DNA sequencing (HTS) of the lymphocyte repertoire (Rep-Seq) represents a promising technology to explore such diversity ex-vivo and assist in the identification of antigen-specific antibodies based on molecular signatures of clonal selection. Therefore, integrative tools for repertoire reconstruction and analysis from antibody sequences are needed. We developed ImmunediveRity, a stand-alone pipeline primarily based in R programming for the integral analysis of B cell repertoire data generated by HTS. The pipeline integrates GNU software and in house scripts to perform quality filtering, sequencing noise correction and repertoire reconstruction based on V, D and J segment as2015-01-01T00:00:00Z20152026-05-05T05:34:03.992Z2019-03-27T02:00:52ZS-EPMC46226552587514010.1080/19420862.2015.1026502