{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(10)"],"submitter":["Zhang MH"],"pubmed_abstract":["<h4>Background</h4>This study observes changes in levels of seminal nitric oxide (NO), nitric oxide synthase (NOS), macrophage migration inhibitory factor (MIF), sperm DNA integrity, chromatin condensation and Caspase-3in adult healthy men after scrotal heat stress (SHS).<h4>Methods</h4>Exposure of the scrotum of 25 healthy male volunteers locally at 40-43°C SHS belt warming 40 min each day for successive 2 d per week. The course of SHS was continuously 3 months. Routine semen analysis, hypo-osmotic swelling (HOS) test, Aniline blue (AB) staining, HOS/AB and terminal deoxynucleotidyl transferase-mediated d UDP nick-end labeling (TUNEL) were carried out before, during and after SHS. Seminal NO and NOS contents were determined by nitrate reduction method. The activated Caspase-3 levels of sp"],"journal":["PloS one"],"pagination":["e0141320"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4626044"],"repository":["biostudies-literature"],"pubmed_title":["Changes in Levels of Seminal Nitric Oxide Synthase, Macrophage Migration Inhibitory Factor, Sperm DNA Integrity and Caspase-3 in Fertile Men after Scrotal Heat Stress."],"pmcid":["PMC4626044"],"pubmed_authors":["Qiu Y","Zhang AD","Wang LG","Zhang MH","Shi ZD"],"additional_accession":[]},"is_claimable":false,"name":"Changes in Levels of Seminal Nitric Oxide Synthase, Macrophage Migration Inhibitory Factor, Sperm DNA Integrity and Caspase-3 in Fertile Men after Scrotal Heat Stress.","description":"<h4>Background</h4>This study observes changes in levels of seminal nitric oxide (NO), nitric oxide synthase (NOS), macrophage migration inhibitory factor (MIF), sperm DNA integrity, chromatin condensation and Caspase-3in adult healthy men after scrotal heat stress (SHS).<h4>Methods</h4>Exposure of the scrotum of 25 healthy male volunteers locally at 40-43°C SHS belt warming 40 min each day for successive 2 d per week. The course of SHS was continuously 3 months. Routine semen analysis, hypo-osmotic swelling (HOS) test, Aniline blue (AB) staining, HOS/AB and terminal deoxynucleotidyl transferase-mediated d UDP nick-end labeling (TUNEL) were carried out before, during and after SHS. Seminal NO and NOS contents were determined by nitrate reduction method. The activated Caspase-3 levels of sp","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015","modification":"2025-04-19T15:27:55.525Z","creation":"2019-03-26T23:28:04Z"},"accession":"S-EPMC4626044","cross_references":{"pubmed":["26512992"],"doi":["10.1371/journal.pone.0141320"]}}