{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(10)"],"submitter":["Sipieter F"],"pubmed_abstract":["Uncoupling of ERK1/2 phosphorylation from subcellular localization is essential towards the understanding of molecular mechanisms that control ERK1/2-mediated cell-fate decision. ERK1/2 non-catalytic functions and discoveries of new specific anchors responsible of the subcellular compartmentalization of ERK1/2 signaling pathway have been proposed as regulation mechanisms for which dynamic monitoring of ERK1/2 localization is necessary. However, studying the spatiotemporal features of ERK2, for instance, in different cellular processes in living cells and tissues requires a tool that can faithfully report on its subcellular distribution. We developed a novel molecular tool, ERK2-LOC, based on the T2A-mediated coexpression of strictly equimolar levels of eGFP-ERK2 and MEK1, to faithfully vis"],"journal":["PloS one"],"pagination":["e0140924"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4627772"],"repository":["biostudies-literature"],"pubmed_title":["Novel Reporter for Faithful Monitoring of ERK2 Dynamics in Living Cells and Model Organisms."],"pmcid":["PMC4627772"],"pubmed_authors":["Riquet FB","Cailliau-Maggio K","Gonzalez Pisfil M","Vandenabeele P","Bodart JF","Heliot L","Spriet C","Sipieter F","Cappe B"],"additional_accession":[]},"is_claimable":false,"name":"Novel Reporter for Faithful Monitoring of ERK2 Dynamics in Living Cells and Model Organisms.","description":"Uncoupling of ERK1/2 phosphorylation from subcellular localization is essential towards the understanding of molecular mechanisms that control ERK1/2-mediated cell-fate decision. ERK1/2 non-catalytic functions and discoveries of new specific anchors responsible of the subcellular compartmentalization of ERK1/2 signaling pathway have been proposed as regulation mechanisms for which dynamic monitoring of ERK1/2 localization is necessary. However, studying the spatiotemporal features of ERK2, for instance, in different cellular processes in living cells and tissues requires a tool that can faithfully report on its subcellular distribution. We developed a novel molecular tool, ERK2-LOC, based on the T2A-mediated coexpression of strictly equimolar levels of eGFP-ERK2 and MEK1, to faithfully vis","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015","modification":"2025-04-19T06:10:42.049Z","creation":"2019-03-26T23:28:06Z"},"accession":"S-EPMC4627772","cross_references":{"pubmed":["26517832"],"doi":["10.1371/journal.pone.0140924"]}}