biostudies-literatureUnknown10(10)Sipieter FUncoupling of ERK1/2 phosphorylation from subcellular localization is essential towards the understanding of molecular mechanisms that control ERK1/2-mediated cell-fate decision. ERK1/2 non-catalytic functions and discoveries of new specific anchors responsible of the subcellular compartmentalization of ERK1/2 signaling pathway have been proposed as regulation mechanisms for which dynamic monitoring of ERK1/2 localization is necessary. However, studying the spatiotemporal features of ERK2, for instance, in different cellular processes in living cells and tissues requires a tool that can faithfully report on its subcellular distribution. We developed a novel molecular tool, ERK2-LOC, based on the T2A-mediated coexpression of strictly equimolar levels of eGFP-ERK2 and MEK1, to faithfully visPloS onee0140924https://www.ebi.ac.uk/biostudies/studies/S-EPMC4627772biostudies-literatureNovel Reporter for Faithful Monitoring of ERK2 Dynamics in Living Cells and Model Organisms.PMC4627772Riquet FBCailliau-Maggio KGonzalez Pisfil MVandenabeele PBodart JFHeliot LSpriet CSipieter FCappe BfalseNovel Reporter for Faithful Monitoring of ERK2 Dynamics in Living Cells and Model Organisms.Uncoupling of ERK1/2 phosphorylation from subcellular localization is essential towards the understanding of molecular mechanisms that control ERK1/2-mediated cell-fate decision. ERK1/2 non-catalytic functions and discoveries of new specific anchors responsible of the subcellular compartmentalization of ERK1/2 signaling pathway have been proposed as regulation mechanisms for which dynamic monitoring of ERK1/2 localization is necessary. However, studying the spatiotemporal features of ERK2, for instance, in different cellular processes in living cells and tissues requires a tool that can faithfully report on its subcellular distribution. We developed a novel molecular tool, ERK2-LOC, based on the T2A-mediated coexpression of strictly equimolar levels of eGFP-ERK2 and MEK1, to faithfully vis2015-01-01T00:00:00Z20152025-04-19T06:10:42.049Z2019-03-26T23:28:06ZS-EPMC46277722651783210.1371/journal.pone.0140924