biostudies-literatureFox JRNIEHS NIH HHS507-519https://www.ebi.ac.uk/biostudies/studies/S-EPMC4628827biostudies-literatureUnknown8(5)Epidemiologic studies have linked diesel exhaust (DE) to cardiovascular and respiratory morbidity and mortality, as well as lung cancer. DE composition is known to vary with many factors, although it is unclear how this influences toxicity. We generated eight DE atmospheres by applying a 2×2×2 factorial design and altering three parameters in a controlled exposure facility: (1) engine load (27 vs 82 %), (2) particle aging (residence time ~5 s vs ~5 min prior to particle collection), and (3) oxidation (with or without ozonation during dilution). Selected exposure concentrations of both diesel exhaust particles (DEPs) and DE gases, DEP oxidative reactivity via DTT activity, and in vitro DEP toxicity in murine endothelial cells were measured for each DE atmosphere. Cell toxicity was assessed Air quality, atmosphere, & healthChemical characterization and in vitro toxicity of diesel exhaust particulate matter generated under varying conditions.PMC4628827T32 ES015459P50 ES015915P30 ES007033T32 ES007032Kavanagh TJCox DPGould TRSheppard LStewart JAKaufman JDLarson TVPaulsen MHDrury BEFox JRSimpson CDfalseChemical characterization and in vitro toxicity of diesel exhaust particulate matter generated under varying conditions.Epidemiologic studies have linked diesel exhaust (DE) to cardiovascular and respiratory morbidity and mortality, as well as lung cancer. DE composition is known to vary with many factors, although it is unclear how this influences toxicity. We generated eight DE atmospheres by applying a 2×2×2 factorial design and altering three parameters in a controlled exposure facility: (1) engine load (27 vs 82 %), (2) particle aging (residence time ~5 s vs ~5 min prior to particle collection), and (3) oxidation (with or without ozonation during dilution). Selected exposure concentrations of both diesel exhaust particles (DEPs) and DE gases, DEP oxidative reactivity via DTT activity, and in vitro DEP toxicity in murine endothelial cells were measured for each DE atmosphere. Cell toxicity was assessed 2015-01-01T00:00:00Z2015 Oct2025-04-04T01:29:29.483Z2019-03-27T02:01:08ZS-EPMC46288272653925410.1007/s11869-014-0301-8