{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Burwitz BJ"],"funding":["amfAR","Creative and Novel Ideas in HIV Research","NIAID NIH HHS","The American Foundation for AIDS Research","Foundation for AIDS and Immune Research","Nonhuman Primate Resource Reagent Program","NIH HHS","PHS HHS","U.S. National Institutes of Health, University of California, San Francisco–Gladstone Institute of Virology and Immunology Center for AIDS Research"],"pagination":["491-501"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4632165"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["96(3)"],"pubmed_abstract":["Nonhuman primates are critical animal models for the study of human disorders and disease and offer a platform to assess the role of immune cells in pathogenesis via depletion of specific cellular subsets. However, this model is currently hindered by the lack of reagents that safely and specifically ablate myeloid cells of the monocyte/macrophage Lin. Given the central importance of macrophages in homeostasis and host immunity, development of a macrophage-depletion technique in nonhuman primates would open new avenues of research. Here, using LA at i.v. doses as low as 0.1 mg/kg, we show a >50% transient depletion of circulating monocytes and tissue-resident macrophages in RMs by an 11-color flow cytometric analysis. Diminution of monocytes was followed rapidly by emigration of monocytes f"],"journal":["Journal of leukocyte biology"],"pubmed_title":["Technical advance: liposomal alendronate depletes monocytes and macrophages in the nonhuman primate model of human disease."],"pmcid":["PMC4632165"],"funding_grant_id":["P30-AI027763","HHSN272200900037C","P30 AI027763","HHSN272200090037C","U24 AI126683","U42 OD010426","OD010976","R24 OD010976","108548","P51 OD011092"],"pubmed_authors":["Ohme MA","Reed JS","Richter Y","Planer SL","Golomb G","Legasse AW","Hammond KB","Burwitz BJ","Sacha JB","Ericsen AJ"],"additional_accession":[]},"is_claimable":false,"name":"Technical advance: liposomal alendronate depletes monocytes and macrophages in the nonhuman primate model of human disease.","description":"Nonhuman primates are critical animal models for the study of human disorders and disease and offer a platform to assess the role of immune cells in pathogenesis via depletion of specific cellular subsets. However, this model is currently hindered by the lack of reagents that safely and specifically ablate myeloid cells of the monocyte/macrophage Lin. Given the central importance of macrophages in homeostasis and host immunity, development of a macrophage-depletion technique in nonhuman primates would open new avenues of research. Here, using LA at i.v. doses as low as 0.1 mg/kg, we show a >50% transient depletion of circulating monocytes and tissue-resident macrophages in RMs by an 11-color flow cytometric analysis. Diminution of monocytes was followed rapidly by emigration of monocytes f","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Sep","modification":"2025-04-22T20:54:15.755Z","creation":"2019-03-27T02:01:18Z"},"accession":"S-EPMC4632165","cross_references":{"pubmed":["24823811"],"doi":["10.1189/jlb.5TA0713-373R","10.1189/jlb.5ta0713-373r"]}}