{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["5"],"submitter":["Jiang C"],"pubmed_abstract":["A label-free, homogenous and sensitive one-step method for the molecular authentication of medicinal snakes has been developed by combining a rapid PCR technique with water-soluble cationic conjugated polyelectrolytes (CCPs). Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were clearly distinguished by the naked eye by utilizing a CCP-based assay in a high-throughput manner. The identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system. In contrast to previous fluorescence-labeled oligonucleotide detection and direct DNA stain hybridization assays, this method does not require designing dye-labeled primers, and unfavorable dimer fluorescence is avoided in this homogenous method."],"journal":["Scientific reports"],"pagination":["16260"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4633673"],"repository":["biostudies-literature"],"pubmed_title":["Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers."],"pmcid":["PMC4633673"],"pubmed_authors":["Hou J","Liu L","Jiang C","Huang L","Jin Y","Yuan Y"],"additional_accession":[]},"is_claimable":false,"name":"Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers.","description":"A label-free, homogenous and sensitive one-step method for the molecular authentication of medicinal snakes has been developed by combining a rapid PCR technique with water-soluble cationic conjugated polyelectrolytes (CCPs). Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were clearly distinguished by the naked eye by utilizing a CCP-based assay in a high-throughput manner. The identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system. In contrast to previous fluorescence-labeled oligonucleotide detection and direct DNA stain hybridization assays, this method does not require designing dye-labeled primers, and unfavorable dimer fluorescence is avoided in this homogenous method.","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Nov","modification":"2024-11-10T08:30:53.098Z","creation":"2019-03-27T02:01:23Z"},"accession":"S-EPMC4633673","cross_references":{"pubmed":["26537289"],"doi":["10.1038/srep16260"]}}