biostudies-literatureYe YFlight Attendant Medical Research InstituteNIAID NIH HHSNational Institutes of HealthNIGMS NIH HHS1816-26https://www.ebi.ac.uk/biostudies/studies/S-EPMC4633763biostudies-literatureUnknown212(11)<h4>Background</h4>Klebsiella pneumoniae causes serious infections and healthcare burdens in humans. We have previously reported that the deficiency of autophagy-related gene (Atg) 7 in macrophages (murine alveolar macrophage cell line [MH-S]) induced irregular host immunity against K. pneumoniae and worsened pathologic effects in the lung. In the current study, we investigated the molecular mechanism by which Atg7 influenced K. pneumoniae-induced inflammatory responses.<h4>Methods</h4>Expression levels of Atg7, ubiquitin (Ub), and tumor necrosis factor (TNF) α and phosphorylation of IκBα (p-IκBα) were determined with immunoblotting. Ubiquitylation of p-IκBα was determined with immunoprecipitation.<h4>Results</h4>We noted an interaction between Atg7 and p-IκBα, which was decreased in MH-S The Journal of infectious diseasesInhibition of p-IκBα Ubiquitylation by Autophagy-Related Gene 7 to Regulate Inflammatory Responses to Bacterial Infection.PMC4633763R15 AI101973-01R01 AI109317R01 AI109317-01A1R03 AI097532-01A1R03 AI097532103007P30 GM103329R15 AI101973Dhasarathy ALi XLichter NWu MJundt MCTan SYe YZhou XHidebrand AfalseInhibition of p-IκBα Ubiquitylation by Autophagy-Related Gene 7 to Regulate Inflammatory Responses to Bacterial Infection.<h4>Background</h4>Klebsiella pneumoniae causes serious infections and healthcare burdens in humans. We have previously reported that the deficiency of autophagy-related gene (Atg) 7 in macrophages (murine alveolar macrophage cell line [MH-S]) induced irregular host immunity against K. pneumoniae and worsened pathologic effects in the lung. In the current study, we investigated the molecular mechanism by which Atg7 influenced K. pneumoniae-induced inflammatory responses.<h4>Methods</h4>Expression levels of Atg7, ubiquitin (Ub), and tumor necrosis factor (TNF) α and phosphorylation of IκBα (p-IκBα) were determined with immunoblotting. Ubiquitylation of p-IκBα was determined with immunoprecipitation.<h4>Results</h4>We noted an interaction between Atg7 and p-IκBα, which was decreased in MH-S 2015-01-01T00:00:00Z2015 Dec2025-05-31T22:54:31.239Z2025-05-31T22:54:31.239ZS-EPMC46337632602244210.1093/infdis/jiv301