{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["5"],"submitter":["Li J"],"pubmed_abstract":["Small heat shock proteins (sHSPs) perform a fundamental role in protecting cells against a wide array of stresses but their biological function during viral infection remains unknown. Rice stripe virus (RSV) causes a severe disease of rice in Eastern Asia. OsHSP20 and its homologue (NbHSP20) were used as baits in yeast two-hybrid (YTH) assays to screen an RSV cDNA library and were found to interact with the viral RNA-dependent RNA polymerase (RdRp) of RSV. Interactions were confirmed by pull-down and BiFC assays. Further analysis showed that the N-terminus (residues 1-296) of the RdRp was crucial for the interaction between the HSP20s and viral RdRp and responsible for the alteration of the sub-cellular localization and distribution pattern of HSP20s in protoplasts of rice and epidermal ce"],"journal":["Scientific reports"],"pagination":["14016"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4642574"],"repository":["biostudies-literature"],"pubmed_title":["Interaction of HSP20 with a viral RdRp changes its sub-cellular localization and distribution pattern in plants."],"pmcid":["PMC4642574"],"pubmed_authors":["Yang J","Li J","Chen JP","Xiang CY","Zhang HM"],"additional_accession":[]},"is_claimable":false,"name":"Interaction of HSP20 with a viral RdRp changes its sub-cellular localization and distribution pattern in plants.","description":"Small heat shock proteins (sHSPs) perform a fundamental role in protecting cells against a wide array of stresses but their biological function during viral infection remains unknown. Rice stripe virus (RSV) causes a severe disease of rice in Eastern Asia. OsHSP20 and its homologue (NbHSP20) were used as baits in yeast two-hybrid (YTH) assays to screen an RSV cDNA library and were found to interact with the viral RNA-dependent RNA polymerase (RdRp) of RSV. Interactions were confirmed by pull-down and BiFC assays. Further analysis showed that the N-terminus (residues 1-296) of the RdRp was crucial for the interaction between the HSP20s and viral RdRp and responsible for the alteration of the sub-cellular localization and distribution pattern of HSP20s in protoplasts of rice and epidermal ce","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Sep","modification":"2026-07-15T20:48:22.623Z","creation":"2019-03-27T02:01:51Z"},"accession":"S-EPMC4642574","cross_references":{"pubmed":["26359114"],"doi":["10.1038/srep14016"]}}