{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Salem JC"],"funding":["NIDDK NIH HHS"],"pagination":["4265-79"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4642859"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(23)"],"pubmed_abstract":["When expressed in epithelial cells, cytohesin-2/ARNO, a guanine nucleotide exchange factor (GEF) for ARF small GTPases, causes a robust migration response. Recent evidence suggests that cytohesin-2/ARNO acts downstream of small the GTPase R-Ras to promote spreading and migration. We hypothesized that cytohesin-2/ARNO could transmit R-Ras signals by regulating the recycling of R-Ras through ARF activation. We found that Eps15-homology domain 1 (EHD1), a protein that associates with the endocytic recycling compartment (ERC), colocalizes with active R-Ras in transiently expressed HeLa cells. In addition, we show that EHD1-positive recycling endosomes are a novel compartment for cytohesin-2/ARNO. Knockdown or expression of GEF-inactive (E156K) cytohesin-2/ARNO causes R-Ras to accumulate on rec"],"journal":["Molecular biology of the cell"],"pubmed_title":["ARF-GEF cytohesin-2/ARNO regulates R-Ras and α5-integrin recycling through an EHD1-positive compartment."],"pmcid":["PMC4642859"],"funding_grant_id":["R01 DK093729"],"pubmed_authors":["Reviriego-Mendoza MM","Salem JC","Santy LC"],"additional_accession":[]},"is_claimable":false,"name":"ARF-GEF cytohesin-2/ARNO regulates R-Ras and α5-integrin recycling through an EHD1-positive compartment.","description":"When expressed in epithelial cells, cytohesin-2/ARNO, a guanine nucleotide exchange factor (GEF) for ARF small GTPases, causes a robust migration response. Recent evidence suggests that cytohesin-2/ARNO acts downstream of small the GTPase R-Ras to promote spreading and migration. We hypothesized that cytohesin-2/ARNO could transmit R-Ras signals by regulating the recycling of R-Ras through ARF activation. We found that Eps15-homology domain 1 (EHD1), a protein that associates with the endocytic recycling compartment (ERC), colocalizes with active R-Ras in transiently expressed HeLa cells. In addition, we show that EHD1-positive recycling endosomes are a novel compartment for cytohesin-2/ARNO. Knockdown or expression of GEF-inactive (E156K) cytohesin-2/ARNO causes R-Ras to accumulate on rec","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Nov","modification":"2025-04-04T13:25:38.617Z","creation":"2019-03-27T02:01:48Z"},"accession":"S-EPMC4642859","cross_references":{"pubmed":["26378252"],"doi":["10.1091/mbc.E15-05-0278"]}}