biostudies-literatureArriaran SPlan Nacional de Ciencia y Tecnología de los AlimentosCIBER-OBN Research WebPlan Nacional de Investigación en Biomedicinae1399https://www.ebi.ac.uk/biostudies/studies/S-EPMC4647552biostudies-literatureUnknown3Background and Objectives. White adipose tissue (WAT) shows marked sex- and diet-dependent differences. However, our metabolic knowledge of WAT, especially on amino acid metabolism, is considerably limited. In the present study, we compared the influence of sex on the amino acid metabolism profile of the four main WAT sites, focused on the paths related to ammonium handling and the urea cycle, as a way to estimate the extent of WAT implication on body amino-nitrogen metabolism. Experimental Design. Adult female and male rats were maintained, undisturbed, under standard conditions for one month. After killing them under isoflurane anesthesia. WAT sites were dissected and weighed. Subcutaneous, perigonadal, retroperitoneal and mesenteric WAT were analyzed for amino acid metabolism gene exprePeerJEffects of sex and site on amino acid metabolism enzyme gene expression and activity in rat white adipose tissue.PMC4647552SAF2012-34895AGL-2011-23635Arriaran SRemesar XFernandez-Lopez JAAlemany MAgnelli SfalseEffects of sex and site on amino acid metabolism enzyme gene expression and activity in rat white adipose tissue.Background and Objectives. White adipose tissue (WAT) shows marked sex- and diet-dependent differences. However, our metabolic knowledge of WAT, especially on amino acid metabolism, is considerably limited. In the present study, we compared the influence of sex on the amino acid metabolism profile of the four main WAT sites, focused on the paths related to ammonium handling and the urea cycle, as a way to estimate the extent of WAT implication on body amino-nitrogen metabolism. Experimental Design. Adult female and male rats were maintained, undisturbed, under standard conditions for one month. After killing them under isoflurane anesthesia. WAT sites were dissected and weighed. Subcutaneous, perigonadal, retroperitoneal and mesenteric WAT were analyzed for amino acid metabolism gene expre2015-01-01T00:00:00Z20152026-05-05T09:12:25.704Z2019-03-27T02:02:04ZS-EPMC46475522658735610.7717/peerj.1399