{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["35(3)"],"submitter":["Sun L"],"pubmed_abstract":["<h4>Aim</h4>To investigate the effects of serum deprivation (SD) on microvesicles (MVs) secreted from human myeloma cells and the implications for disease progression.<h4>Methods</h4>RPMI 8226, U266, and KM3 human myeloma cells were incubated in medium containing 10% (non-SD) or 1% fetal bovine serum (SD) and MVs were isolated. The levels and size distribution of MVs were analyzed with flow cytometry. The protein profiles of MVs were studied using 2D SDS-PAGE, MALDI-TOF-MS, and Western blotting. NF-κB activation was analyzed using EMSA. Angiogenesis was examined in Eahy926 endothelial cells.<h4>Results</h4>Exposure of RPMI 8226 cells to SD for 24 h did not alter the number of apoptotic cells. However, SD increased the number of MVs from RPMI 8226, U266, and KM3 cells to 2.5-, 4.3-, and 3.8"],"journal":["Acta pharmacologica Sinica"],"pagination":["381-93"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4647891"],"repository":["biostudies-literature"],"pubmed_title":["Serum deprivation elevates the levels of microvesicles with different size distributions and selectively enriched proteins in human myeloma cells in vitro."],"pmcid":["PMC4647891"],"pubmed_authors":["Sun L","Chen ZC","Wu PH","Chen WQ","Zhu XJ","Zou P","Li QB","Wang HX"],"additional_accession":[]},"is_claimable":false,"name":"Serum deprivation elevates the levels of microvesicles with different size distributions and selectively enriched proteins in human myeloma cells in vitro.","description":"<h4>Aim</h4>To investigate the effects of serum deprivation (SD) on microvesicles (MVs) secreted from human myeloma cells and the implications for disease progression.<h4>Methods</h4>RPMI 8226, U266, and KM3 human myeloma cells were incubated in medium containing 10% (non-SD) or 1% fetal bovine serum (SD) and MVs were isolated. The levels and size distribution of MVs were analyzed with flow cytometry. The protein profiles of MVs were studied using 2D SDS-PAGE, MALDI-TOF-MS, and Western blotting. NF-κB activation was analyzed using EMSA. Angiogenesis was examined in Eahy926 endothelial cells.<h4>Results</h4>Exposure of RPMI 8226 cells to SD for 24 h did not alter the number of apoptotic cells. However, SD increased the number of MVs from RPMI 8226, U266, and KM3 cells to 2.5-, 4.3-, and 3.8","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Mar","modification":"2025-04-22T15:10:37.154Z","creation":"2019-03-27T02:02:05Z"},"accession":"S-EPMC4647891","cross_references":{"pubmed":["24374813"],"doi":["10.1038/aps.2013.166"]}}