{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(11)"],"submitter":["Catherine C"],"pubmed_abstract":["Progress towards genetic sequencing of human parasites has provided the groundwork for a post-genomic approach to develop novel antigens for the diagnosis and treatment of parasite infections. To fully utilize the genomic data, however, high-throughput methodologies are required for functional analysis of the proteins encoded in the genomic sequences. In this study, we investigated cell-free expression and in situ immobilization of parasite proteins as a novel platform for the discovery of antigenic proteins. PCR-amplified parasite DNA was immobilized on microbeads that were also functionalized to capture synthesized proteins. When the microbeads were incubated in a reaction mixture for cell-free synthesis, proteins expressed from the microbead-immobilized DNA were instantly immobilized on"],"journal":["PloS one"],"pagination":["e0143597"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4657965"],"repository":["biostudies-literature"],"pubmed_title":["Cell-Free Expression and In Situ Immobilization of Parasite Proteins from Clonorchis sinensis for Rapid Identification of Antigenic Candidates."],"pmcid":["PMC4657965"],"pubmed_authors":["Catherine C","Shin HI","Lee SW","Kim YJ","Kim HC","Ju JW","Kim DM"],"additional_accession":[]},"is_claimable":false,"name":"Cell-Free Expression and In Situ Immobilization of Parasite Proteins from Clonorchis sinensis for Rapid Identification of Antigenic Candidates.","description":"Progress towards genetic sequencing of human parasites has provided the groundwork for a post-genomic approach to develop novel antigens for the diagnosis and treatment of parasite infections. To fully utilize the genomic data, however, high-throughput methodologies are required for functional analysis of the proteins encoded in the genomic sequences. In this study, we investigated cell-free expression and in situ immobilization of parasite proteins as a novel platform for the discovery of antigenic proteins. PCR-amplified parasite DNA was immobilized on microbeads that were also functionalized to capture synthesized proteins. When the microbeads were incubated in a reaction mixture for cell-free synthesis, proteins expressed from the microbead-immobilized DNA were instantly immobilized on","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015","modification":"2025-04-19T08:20:10.464Z","creation":"2019-03-26T22:56:19Z"},"accession":"S-EPMC4657965","cross_references":{"pubmed":["26599101"],"doi":["10.1371/journal.pone.0143597"]}}