{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gane JM"],"funding":["West Midlands Chest Fund","Medical Research Council","Academy of Medical Sciences"],"pagination":["20"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4665865"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14"],"pubmed_abstract":["<h4>Background</h4>Polymorphisms in the TNF-A gene have been associated with chronic obstructive pulmonary disease (COPD) in some case-control studies. Previous work has shown that COPD/chronic bronchitis subjects with alpha-1 antitrypsin deficiency with the rs361525 TNF-α single nucleotide polymorphism have 100 times more TNF-in spontaneous sputum than disease matched controls. Our objective was to determine if the presence of this polymorphism increased TNF-α production by blood monocytes from COPD subjects.<h4>Findings</h4>Monocytes from 18 COPD/alpha-1 antitrypsin deficient subjects, with and without the rs361525 polymorphism, were cultured in the presence or absence of lipopolysaccharide. Cell-free supernatants were analyzed by ELISA and real-time PCR performed using cDNA from extract"],"journal":["Journal of negative results in biomedicine"],"pubmed_title":["The rs361525 polymorphism does not increase production of tumor necrosis factor alpha by monocytes from alpha-1 antitrypsin deficient subjects with chronic obstructive pulmonary disease - a pilot study."],"pmcid":["PMC4665865"],"funding_grant_id":["AMS-SGCL5-Sapey","002013","MR/L008335/1"],"pubmed_authors":["Sapey E","Gane JM","Stockley RA"],"additional_accession":[]},"is_claimable":false,"name":"The rs361525 polymorphism does not increase production of tumor necrosis factor alpha by monocytes from alpha-1 antitrypsin deficient subjects with chronic obstructive pulmonary disease - a pilot study.","description":"<h4>Background</h4>Polymorphisms in the TNF-A gene have been associated with chronic obstructive pulmonary disease (COPD) in some case-control studies. Previous work has shown that COPD/chronic bronchitis subjects with alpha-1 antitrypsin deficiency with the rs361525 TNF-α single nucleotide polymorphism have 100 times more TNF-in spontaneous sputum than disease matched controls. Our objective was to determine if the presence of this polymorphism increased TNF-α production by blood monocytes from COPD subjects.<h4>Findings</h4>Monocytes from 18 COPD/alpha-1 antitrypsin deficient subjects, with and without the rs361525 polymorphism, were cultured in the presence or absence of lipopolysaccharide. Cell-free supernatants were analyzed by ELISA and real-time PCR performed using cDNA from extract","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Dec","modification":"2025-04-19T01:39:03.735Z","creation":"2019-03-27T02:03:02Z"},"accession":"S-EPMC4665865","cross_references":{"pubmed":["26620975"],"doi":["10.1186/s12952-015-0039-3"]}}