biostudies-literatureMoreira TGNIAID NIH HHSNCI NIH HHSNIGMS NIH HHS17655https://www.ebi.ac.uk/biostudies/studies/S-EPMC4668385biostudies-literatureUnknown5The Sec13 protein functions in various intracellular compartments including the nuclear pore complex, COPII-coated vesicles, and inside the nucleus as a transcription regulator. Here we developed a mouse model that expresses low levels of Sec13 (Sec13(H/-)) to assess its functions in vivo, as Sec13 knockout is lethal. These Sec13 mutant mice did not present gross defects in anatomy and physiology. However, the reduced levels of Sec13 in vivo yielded specific immunological defects. In particular, these Sec13 mutant mice showed low levels of MHC I and II expressed by macrophages, low levels of INF-γ and IL-6 expressed by stimulated T cells, and low frequencies of splenic IFN-γ+CD8+ T cells. In contrast, the levels of soluble and membrane-bound TGF-β as well as serum immunoglobulin productionScientific reportsSec13 Regulates Expression of Specific Immune Factors Involved in Inflammation In Vivo.PMC4668385R01 AI079110R01 GM096070R01 GM113874-01R01 GM113874GM096070P30 CA142543R01 AI089539Shelton JYang JShaulov LWilliams JSakthivel RNussenzveig DRZhang LKuss SKMoreira TGSomatilaka BFaria AMSeemann JFontoura BMHarel AfalseSec13 Regulates Expression of Specific Immune Factors Involved in Inflammation In Vivo.The Sec13 protein functions in various intracellular compartments including the nuclear pore complex, COPII-coated vesicles, and inside the nucleus as a transcription regulator. Here we developed a mouse model that expresses low levels of Sec13 (Sec13(H/-)) to assess its functions in vivo, as Sec13 knockout is lethal. These Sec13 mutant mice did not present gross defects in anatomy and physiology. However, the reduced levels of Sec13 in vivo yielded specific immunological defects. In particular, these Sec13 mutant mice showed low levels of MHC I and II expressed by macrophages, low levels of INF-γ and IL-6 expressed by stimulated T cells, and low frequencies of splenic IFN-γ+CD8+ T cells. In contrast, the levels of soluble and membrane-bound TGF-β as well as serum immunoglobulin production2015-01-01T00:00:00Z2015 Dec2025-04-04T14:06:26.448Z2019-03-27T02:04:32ZS-EPMC46683852663197210.1038/srep17655