biostudies-literatureUnknown5Wang RAs an important part of synthetic biology, synthetic promoter has gradually become a hotspot in current biology. The purposes of the present study were to synthesize green tissue-specific promoters and to discover green tissue-specific cis-elements. We first assembled several regulatory sequences related to tissue-specific expression in different combinations, aiming to obtain novel green tissue-specific synthetic promoters. GUS assays of the transgenic plants indicated 5 synthetic promoters showed green tissue-specific expression patterns and different expression efficiencies in various tissues. Subsequently, we scanned and counted the cis-elements in different tissue-specific promoters based on the plant cis-elements database PLACE and the rice cDNA microarray database CREP for green tisScientific reports18256https://www.ebi.ac.uk/biostudies/studies/S-EPMC4676006biostudies-literatureNovel green tissue-specific synthetic promoters and cis-regulatory elements in rice.PMC4676006Zhu MLiu ZWang RYe RLin YZhou FChen HfalseNovel green tissue-specific synthetic promoters and cis-regulatory elements in rice.As an important part of synthetic biology, synthetic promoter has gradually become a hotspot in current biology. The purposes of the present study were to synthesize green tissue-specific promoters and to discover green tissue-specific cis-elements. We first assembled several regulatory sequences related to tissue-specific expression in different combinations, aiming to obtain novel green tissue-specific synthetic promoters. GUS assays of the transgenic plants indicated 5 synthetic promoters showed green tissue-specific expression patterns and different expression efficiencies in various tissues. Subsequently, we scanned and counted the cis-elements in different tissue-specific promoters based on the plant cis-elements database PLACE and the rice cDNA microarray database CREP for green tis2015-01-01T00:00:00Z2015 Dec2025-04-21T19:26:38.068Z2019-03-27T02:04:59ZS-EPMC46760062665567910.1038/srep18256