{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15"],"submitter":["de Vries S"],"pubmed_abstract":["Heterotrimeric G-proteins are important signalling switches, present in all eukaryotic kingdoms. In plants they regulate several developmental functions and play an important role in plant-microbe interactions. The current knowledge on plant G-proteins is mostly based on model angiosperms and little is known about the G-protein repertoire and function in other lineages. In this study we investigate the heterotrimeric G-protein subunit repertoire in Pinaceae, including phylogenetic relationships, radiation and sequence diversity levels in relation to other plant linages. We also investigate functional diversification of the G-protein complex in Picea abies by analysing transcriptional regulation of the G-protein subunits in different tissues and in response to pathogen infection.A full repertoire of G-protein subunits in several conifer species were identified in silico. The full-length P. abies coding regions of one G?-, one G?- and four G?-subunits were cloned and sequenced. The phylogenetic analysis of the G?-subunits showed that PaGG1 clustered with A-type-like subunits, PaGG3 and PaGG4 clustered with C-type-like subunits, while PaGG2 and its orthologs represented a novel conifer-specific putative G?-subunit type. Gene expression analyses by quantitative PCR of P. abies G-protein subunits showed specific up-regulation of the G?-subunit gene PaGPA1 and the G?-subunit gene PaGG1 in response to Heterobasidion annosum sensu lato infection.Conifers possess a full repertoire of G-protein subunits. The differential regulation of PaGPA1 and PaGG1 indicates that the heterotrimeric G-protein complex represents a critical linchpin in Heterobasidion annosum s.l. perception and downstream signaling in P. abies."],"journal":["BMC plant biology"],"pagination":["287"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4676809"],"repository":["biostudies-literature"],"pubmed_title":["Heterotrimeric G-proteins in Picea abies and their regulation in response to Heterobasidion annosum s.l. infection."],"pmcid":["PMC4676809"],"pubmed_authors":["de Vries S","Blair PB","Elfstrand M","Karlsson M","Mukhtar MS","Nemesio-Gorriz M"],"additional_accession":[]},"is_claimable":false,"name":"Heterotrimeric G-proteins in Picea abies and their regulation in response to Heterobasidion annosum s.l. infection.","description":"Heterotrimeric G-proteins are important signalling switches, present in all eukaryotic kingdoms. In plants they regulate several developmental functions and play an important role in plant-microbe interactions. The current knowledge on plant G-proteins is mostly based on model angiosperms and little is known about the G-protein repertoire and function in other lineages. In this study we investigate the heterotrimeric G-protein subunit repertoire in Pinaceae, including phylogenetic relationships, radiation and sequence diversity levels in relation to other plant linages. We also investigate functional diversification of the G-protein complex in Picea abies by analysing transcriptional regulation of the G-protein subunits in different tissues and in response to pathogen infection.A full repertoire of G-protein subunits in several conifer species were identified in silico. The full-length P. abies coding regions of one G?-, one G?- and four G?-subunits were cloned and sequenced. The phylogenetic analysis of the G?-subunits showed that PaGG1 clustered with A-type-like subunits, PaGG3 and PaGG4 clustered with C-type-like subunits, while PaGG2 and its orthologs represented a novel conifer-specific putative G?-subunit type. Gene expression analyses by quantitative PCR of P. abies G-protein subunits showed specific up-regulation of the G?-subunit gene PaGPA1 and the G?-subunit gene PaGG1 in response to Heterobasidion annosum sensu lato infection.Conifers possess a full repertoire of G-protein subunits. The differential regulation of PaGPA1 and PaGG1 indicates that the heterotrimeric G-protein complex represents a critical linchpin in Heterobasidion annosum s.l. perception and downstream signaling in P. abies.","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015","modification":"2020-11-19T08:33:48Z","creation":"2019-06-06T15:11:48Z"},"accession":"S-EPMC4676809","cross_references":{"pubmed":["26654722"],"doi":["10.1186/s12870-015-0676-1"]}}