{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["5"],"submitter":["Taleahmad S"],"pubmed_abstract":["The differentiation potential of pluripotent embryonic stem cells (ESCs) can be manipulated via serum and medium conditions for direct cellular development or to maintain a naïve ground state. The self-renewal state of ESCs can thus be induced by adding inhibitors of mitogen activated protein kinase (MAPK) and glycogen synthase kinase-3 (Gsk3), known as 2 inhibitors (2i) treatment. We have used a shotgun proteomics approach to investigate differences in protein expressions between 2i- and serum-grown mESCs. The results indicated that 164 proteins were significantly upregulated and 107 proteins downregulated in 2i-grown cells compared to serum. Protein pathways in 2i-grown cells with the highest enrichment were associated with glycolysis and gluconeogenesis. Protein pathways related to orga"],"journal":["Scientific reports"],"pagination":["17985"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4680864"],"repository":["biostudies-literature"],"pubmed_title":["Proteome Analysis of Ground State Pluripotency."],"pmcid":["PMC4680864"],"pubmed_authors":["Taleahmad S","Mollamohammadi S","Mirzaei M","Salekdeh GH","Sharifi-Zarchi A","Hassani SN","Baharvand H","Haynes PA","Parker LM"],"additional_accession":[]},"is_claimable":false,"name":"Proteome Analysis of Ground State Pluripotency.","description":"The differentiation potential of pluripotent embryonic stem cells (ESCs) can be manipulated via serum and medium conditions for direct cellular development or to maintain a naïve ground state. The self-renewal state of ESCs can thus be induced by adding inhibitors of mitogen activated protein kinase (MAPK) and glycogen synthase kinase-3 (Gsk3), known as 2 inhibitors (2i) treatment. We have used a shotgun proteomics approach to investigate differences in protein expressions between 2i- and serum-grown mESCs. The results indicated that 164 proteins were significantly upregulated and 107 proteins downregulated in 2i-grown cells compared to serum. Protein pathways in 2i-grown cells with the highest enrichment were associated with glycolysis and gluconeogenesis. Protein pathways related to orga","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Dec","modification":"2025-04-19T08:20:22.022Z","creation":"2019-03-27T02:05:14Z"},"accession":"S-EPMC4680864","cross_references":{"pubmed":["26671762"],"doi":["10.1038/srep17985"]}}