biostudies-literatureUnknown5Taleahmad SThe differentiation potential of pluripotent embryonic stem cells (ESCs) can be manipulated via serum and medium conditions for direct cellular development or to maintain a naïve ground state. The self-renewal state of ESCs can thus be induced by adding inhibitors of mitogen activated protein kinase (MAPK) and glycogen synthase kinase-3 (Gsk3), known as 2 inhibitors (2i) treatment. We have used a shotgun proteomics approach to investigate differences in protein expressions between 2i- and serum-grown mESCs. The results indicated that 164 proteins were significantly upregulated and 107 proteins downregulated in 2i-grown cells compared to serum. Protein pathways in 2i-grown cells with the highest enrichment were associated with glycolysis and gluconeogenesis. Protein pathways related to orgaScientific reports17985https://www.ebi.ac.uk/biostudies/studies/S-EPMC4680864biostudies-literatureProteome Analysis of Ground State Pluripotency.PMC4680864Taleahmad SMollamohammadi SMirzaei MSalekdeh GHSharifi-Zarchi AHassani SNBaharvand HHaynes PAParker LMfalseProteome Analysis of Ground State Pluripotency.The differentiation potential of pluripotent embryonic stem cells (ESCs) can be manipulated via serum and medium conditions for direct cellular development or to maintain a naïve ground state. The self-renewal state of ESCs can thus be induced by adding inhibitors of mitogen activated protein kinase (MAPK) and glycogen synthase kinase-3 (Gsk3), known as 2 inhibitors (2i) treatment. We have used a shotgun proteomics approach to investigate differences in protein expressions between 2i- and serum-grown mESCs. The results indicated that 164 proteins were significantly upregulated and 107 proteins downregulated in 2i-grown cells compared to serum. Protein pathways in 2i-grown cells with the highest enrichment were associated with glycolysis and gluconeogenesis. Protein pathways related to orga2015-01-01T00:00:00Z2015 Dec2025-04-19T08:20:22.022Z2019-03-27T02:05:14ZS-EPMC46808642667176210.1038/srep17985