biostudies-literatureVarmeh SNIDDK NIH HHSNCI NIH HHS152-62https://www.ebi.ac.uk/biostudies/studies/S-EPMC4688239biostudies-literatureUnknown159(1)<h4>Background</h4>Investigating BRAF((V600E)) inhibitors (BRAFi) as a strategy to treat patients with aggressive thyroid tumors harboring the BRAF((V600E)) mutant currently is in progress, and drug resistance is expected to pose a challenge. MicroRNAs (miRNAs) are involved in development of resistance to a variety of drugs in different malignancies.<h4>Methods</h4>miRNA expression profiles in the human anaplastic thyroid cancer cell line (8505c) were compared with its PLX4720-resistant counterpart (8505c-R) by the use of Illumina deep sequencing. We conducted a functional annotation and pathway analysis of the putative and experimentally validated target genes of the significantly altered miRNAs.<h4>Results</h4>We identified 61 known and 2 novel miRNAs whose expression was altered greatlySurgeryGenome-wide analysis of differentially expressed miRNA in PLX4720-resistant and parental human thyroid cancer cell lines.PMC4688239P30 DK040561R01 CA1497381R01CA149738-01A1Varmeh SSadreyev RIBrauner EHolm TVanden Borre PWang YParangi SGunda VfalseGenome-wide analysis of differentially expressed miRNA in PLX4720-resistant and parental human thyroid cancer cell lines.<h4>Background</h4>Investigating BRAF((V600E)) inhibitors (BRAFi) as a strategy to treat patients with aggressive thyroid tumors harboring the BRAF((V600E)) mutant currently is in progress, and drug resistance is expected to pose a challenge. MicroRNAs (miRNAs) are involved in development of resistance to a variety of drugs in different malignancies.<h4>Methods</h4>miRNA expression profiles in the human anaplastic thyroid cancer cell line (8505c) were compared with its PLX4720-resistant counterpart (8505c-R) by the use of Illumina deep sequencing. We conducted a functional annotation and pathway analysis of the putative and experimentally validated target genes of the significantly altered miRNAs.<h4>Results</h4>We identified 61 known and 2 novel miRNAs whose expression was altered greatly2016-01-01T00:00:00Z2016 Jan2025-04-03T22:41:26.081Z2019-03-27T02:05:40ZS-EPMC46882392645612410.1016/j.surg.2015.06.046