{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yang S"],"funding":["NHLBI NIH HHS","NIAMS NIH HHS"],"pagination":["45-54"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4696882"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["193(1)"],"pubmed_abstract":["Single particle analysis is widely used for three-dimensional reconstruction of helical filaments. Near-atomic resolution has been obtained for several well-ordered filaments. However, it is still a challenge to achieve high resolution for filaments with flexible subunits and a large axial rise per subunit relative to pixel size. Here, we describe an approach that improves the resolution in such cases. In filaments with a large axial rise, many segments must be shifted a long distance along the filament axis to match with a reference projection, potentially causing loss of alignment accuracy and hence resolution. In our study of myosin filaments, we overcame this problem by pre-determining the axial positions of myosin head crowns within segments to decrease the alignment error. In additio"],"journal":["Journal of structural biology"],"pubmed_title":["An approach to improve the resolution of helical filaments with a large axial rise and flexible subunits."],"pmcid":["PMC4696882"],"funding_grant_id":["R01 AR034711","P01 HL059408","R01 AR067279","R01 AR062279"],"pubmed_authors":["Craig R","Yang S","Woodhead JL","Zhao FQ","Sulbaran G"],"additional_accession":[]},"is_claimable":false,"name":"An approach to improve the resolution of helical filaments with a large axial rise and flexible subunits.","description":"Single particle analysis is widely used for three-dimensional reconstruction of helical filaments. Near-atomic resolution has been obtained for several well-ordered filaments. However, it is still a challenge to achieve high resolution for filaments with flexible subunits and a large axial rise per subunit relative to pixel size. Here, we describe an approach that improves the resolution in such cases. In filaments with a large axial rise, many segments must be shifted a long distance along the filament axis to match with a reference projection, potentially causing loss of alignment accuracy and hence resolution. In our study of myosin filaments, we overcame this problem by pre-determining the axial positions of myosin head crowns within segments to decrease the alignment error. In additio","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Jan","modification":"2026-05-05T08:09:25.578Z","creation":"2019-03-27T02:06:02Z"},"accession":"S-EPMC4696882","cross_references":{"pubmed":["26592473"],"doi":["10.1016/j.jsb.2015.11.007"]}}