biostudies-literatureEmerson JMHHS | NIH | National Institute of General Medical SciencesNIGMS NIH HHS15707-12https://www.ebi.ac.uk/biostudies/studies/S-EPMC4697410biostudies-literatureUnknown112(51)Mutants in the period-1 (prd-1) gene, characterized by a recessive allele, display a reduced growth rate and period lengthening of the developmental cycle controlled by the circadian clock. We refined the genetic location of prd-1 and used whole genome sequencing to find the mutation defining it, confirming the identity of prd-1 by rescuing the mutant circadian phenotype via transformation. PRD-1 is an RNA helicase whose orthologs, DDX5 [DEAD (Asp-Glu-Ala-Asp) Box Helicase 5] and DDX17 in humans and DBP2 (Dead Box Protein 2) in yeast, are implicated in various processes, including transcriptional regulation, elongation, and termination, ribosome biogenesis, and mRNA decay. Although prd-1 mutants display a long period (∼25 h) circadian developmental cycle, they interestingly display a WT peProceedings of the National Academy of Sciences of the United States of Americaperiod-1 encodes an ATP-dependent RNA helicase that influences nutritional compensation of the Neurospora circadian clock.PMC4697410R01 GM034985R01 GM083336GM34985GM083336R37 GM034985R35 GM118021Emerson JMDunlap JCRingelberg CSLoros JJBartholomai BMBaker SEfalseperiod-1 encodes an ATP-dependent RNA helicase that influences nutritional compensation of the Neurospora circadian clock.Mutants in the period-1 (prd-1) gene, characterized by a recessive allele, display a reduced growth rate and period lengthening of the developmental cycle controlled by the circadian clock. We refined the genetic location of prd-1 and used whole genome sequencing to find the mutation defining it, confirming the identity of prd-1 by rescuing the mutant circadian phenotype via transformation. PRD-1 is an RNA helicase whose orthologs, DDX5 [DEAD (Asp-Glu-Ala-Asp) Box Helicase 5] and DDX17 in humans and DBP2 (Dead Box Protein 2) in yeast, are implicated in various processes, including transcriptional regulation, elongation, and termination, ribosome biogenesis, and mRNA decay. Although prd-1 mutants display a long period (∼25 h) circadian developmental cycle, they interestingly display a WT pe2015-01-01T00:00:00Z2015 Dec2025-04-19T17:51:59.398Z2019-08-04T07:55:56ZS-EPMC46974102664718410.1073/pnas.1521918112