{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang L"],"funding":["Intramural NIH HHS","NIDA NIH HHS"],"pagination":["12777-81"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4698351"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["54(43)"],"pubmed_abstract":["Azido (18) F-arenes are important and versatile building blocks for the radiolabeling of biomolecules via Huisgen cycloaddition (\"click chemistry\") for positron emission tomography (PET). However, routine access to such clickable agents is challenged by inefficient and/or poorly defined multistep radiochemical approaches. A high-yielding direct radiofluorination for azido (18) F-arenes was achieved through the development of an ortho-oxygen-stabilized iodonium derivative (OID). This OID strategy addresses an unmet need for a reliable azido (18) F-arene clickable agent for bioconjugation reactions. A ssDNA aptamer was radiolabeled with this agent and visualized in a xenograft mouse model of human colon cancer by PET, which demonstrates that this OID approach is a convenient and highly efficient way of labeling and tracking biomolecules."],"journal":["Angewandte Chemie (International ed. in English)"],"pubmed_title":["Ortho-Stabilized (18) F-Azido Click Agents and their Application in PET Imaging with Single-Stranded DNA Aptamers."],"pmcid":["PMC4698351"],"funding_grant_id":["ZIA EB000073-06","K01 DA038000","Z99 EB999999"],"pubmed_authors":["Weiss ID","Liang SH","Vasdev N","Jacobson O","Collier L","Chen X","Rotstein BH","Avdic D","Wang L"],"additional_accession":[]},"is_claimable":false,"name":"Ortho-Stabilized (18) F-Azido Click Agents and their Application in PET Imaging with Single-Stranded DNA Aptamers.","description":"Azido (18) F-arenes are important and versatile building blocks for the radiolabeling of biomolecules via Huisgen cycloaddition (\"click chemistry\") for positron emission tomography (PET). However, routine access to such clickable agents is challenged by inefficient and/or poorly defined multistep radiochemical approaches. A high-yielding direct radiofluorination for azido (18) F-arenes was achieved through the development of an ortho-oxygen-stabilized iodonium derivative (OID). This OID strategy addresses an unmet need for a reliable azido (18) F-arene clickable agent for bioconjugation reactions. A ssDNA aptamer was radiolabeled with this agent and visualized in a xenograft mouse model of human colon cancer by PET, which demonstrates that this OID approach is a convenient and highly efficient way of labeling and tracking biomolecules.","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Oct","modification":"2020-10-31T08:10:46Z","creation":"2019-03-27T02:06:08Z"},"accession":"S-EPMC4698351","cross_references":{"pubmed":["26308650"],"doi":["10.1002/anie.201505927"]}}