{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bernardo D"],"funding":["Worldwide Cancer Research","NIDDK NIH HHS","Wellcome Trust","Biotechnology and Biological Sciences Research Council"],"pagination":["22-39.e5"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4705905"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2(1)"],"pubmed_abstract":["<h4>Background & aims</h4>Most knowledge about gastrointestinal (GI)-tract dendritic cells (DC) relies on murine studies where CD103<sup>+</sup> DC specialize in generating immune tolerance with the functionality of CD11b<sup>+/-</sup> subsets being unclear. Information about human GI-DC is scarce, especially regarding regional specifications. Here, we characterized human DC properties throughout the human colon.<h4>Methods</h4>Paired proximal (right/ascending) and distal (left/descending) human colonic biopsies from 95 healthy subjects were taken; DC were assessed by flow cytometry and microbiota composition assessed by 16S rRNA gene sequencing.<h4>Results</h4>Colonic DC identified were myeloid (mDC, CD11c<sup>+</sup>CD123<sup>-</sup>) and further divided based on CD103 and SIRPα (human a"],"journal":["Cellular and molecular gastroenterology and hepatology"],"pubmed_title":["Chemokine (C-C Motif) Receptor 2 Mediates Dendritic Cell Recruitment to the Human Colon but Is Not Responsible for Differences Observed in Dendritic Cell Subsets, Phenotype, and Function Between the Proximal and Distal Colon."],"pmcid":["PMC4705905"],"funding_grant_id":["T32 DK007632","098051","12-0234","BBS/E/F/00044446"],"pubmed_authors":["Man R","Fernandez-Salazar L","Scott P","English NR","Carding SR","Knight SC","Durant L","Walker AW","Reddi D","Hart AL","Siaw YH","Paulsen M","Mann ER","Bernardo D","Hendy P","Parkhill J","Garrote JA","Malietzis G","Landy J","Bayiroglu F","Vora R","Bassity E","Peake ST","Arranz E","Sanchez-Recio E","Phillips RK","Montalvillo E","Lee GH","Murugananthan AU","Al-Hassi HO"],"additional_accession":[]},"is_claimable":false,"name":"Chemokine (C-C Motif) Receptor 2 Mediates Dendritic Cell Recruitment to the Human Colon but Is Not Responsible for Differences Observed in Dendritic Cell Subsets, Phenotype, and Function Between the Proximal and Distal Colon.","description":"<h4>Background & aims</h4>Most knowledge about gastrointestinal (GI)-tract dendritic cells (DC) relies on murine studies where CD103<sup>+</sup> DC specialize in generating immune tolerance with the functionality of CD11b<sup>+/-</sup> subsets being unclear. Information about human GI-DC is scarce, especially regarding regional specifications. Here, we characterized human DC properties throughout the human colon.<h4>Methods</h4>Paired proximal (right/ascending) and distal (left/descending) human colonic biopsies from 95 healthy subjects were taken; DC were assessed by flow cytometry and microbiota composition assessed by 16S rRNA gene sequencing.<h4>Results</h4>Colonic DC identified were myeloid (mDC, CD11c<sup>+</sup>CD123<sup>-</sup>) and further divided based on CD103 and SIRPα (human a","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Jan","modification":"2025-04-18T22:02:56.433Z","creation":"2019-06-06T15:16:47Z"},"accession":"S-EPMC4705905","cross_references":{"pubmed":["26866054"],"doi":["10.1016/j.jcmgh.2015.08.006"]}}