biostudies-literatureBernardo DWorldwide Cancer ResearchNIDDK NIH HHSWellcome TrustBiotechnology and Biological Sciences Research Council22-39.e5https://www.ebi.ac.uk/biostudies/studies/S-EPMC4705905biostudies-literatureUnknown2(1)<h4>Background & aims</h4>Most knowledge about gastrointestinal (GI)-tract dendritic cells (DC) relies on murine studies where CD103⁺ DC specialize in generating immune tolerance with the functionality of CD11b^+/- subsets being unclear. Information about human GI-DC is scarce, especially regarding regional specifications. Here, we characterized human DC properties throughout the human colon.<h4>Methods</h4>Paired proximal (right/ascending) and distal (left/descending) human colonic biopsies from 95 healthy subjects were taken; DC were assessed by flow cytometry and microbiota composition assessed by 16S rRNA gene sequencing.<h4>Results</h4>Colonic DC identified were myeloid (mDC, CD11c⁺CD123^-) and further divided based on CD103 and SIRPα (human aCellular and molecular gastroenterology and hepatologyChemokine (C-C Motif) Receptor 2 Mediates Dendritic Cell Recruitment to the Human Colon but Is Not Responsible for Differences Observed in Dendritic Cell Subsets, Phenotype, and Function Between the Proximal and Distal Colon.PMC4705905T32 DK00763209805112-0234BBS/E/F/00044446Man RFernandez-Salazar LScott PEnglish NRCarding SRKnight SCDurant LWalker AWReddi DHart ALSiaw YHPaulsen MMann ERBernardo DHendy PParkhill JGarrote JAMalietzis GLandy JBayiroglu FVora RBassity EPeake STArranz ESanchez-Recio EPhillips RKMontalvillo ELee GHMurugananthan AUAl-Hassi HOfalseChemokine (C-C Motif) Receptor 2 Mediates Dendritic Cell Recruitment to the Human Colon but Is Not Responsible for Differences Observed in Dendritic Cell Subsets, Phenotype, and Function Between the Proximal and Distal Colon.<h4>Background & aims</h4>Most knowledge about gastrointestinal (GI)-tract dendritic cells (DC) relies on murine studies where CD103⁺ DC specialize in generating immune tolerance with the functionality of CD11b^+/- subsets being unclear. Information about human GI-DC is scarce, especially regarding regional specifications. Here, we characterized human DC properties throughout the human colon.<h4>Methods</h4>Paired proximal (right/ascending) and distal (left/descending) human colonic biopsies from 95 healthy subjects were taken; DC were assessed by flow cytometry and microbiota composition assessed by 16S rRNA gene sequencing.<h4>Results</h4>Colonic DC identified were myeloid (mDC, CD11c⁺CD123^-) and further divided based on CD103 and SIRPα (human a2016-01-01T00:00:00Z2016 Jan2025-04-18T22:02:56.433Z2019-06-06T15:16:47ZS-EPMC47059052686605410.1016/j.jcmgh.2015.08.006