{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Leriche G"],"funding":["NIA NIH HHS","National Institute on Aging","Air Force Office of Scientific Research"],"pagination":["40-5"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4720558"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7(1)"],"pubmed_abstract":["The ADAM family of metalloproteases cleave a diverse range of transmembrane substrates, resulting in the release of their soluble ectodomains. This process of protein shedding, termed α-secretase processing, is involved in many facets of both normal and disease related cellular function. While the processing of substrates has been well documented, the regulation and trafficking of the ADAMs are less well understood. Tools that allow for the study of ADAMs under their native environment will allow for a better understanding of their regulation and activity. Here we describe the design and evaluation of a novel fluorescent analogue of a well-characterized ADAM inhibitor, Batimastat. This probe exhibited similar activity for inhibiting α-secretase processing in cells as did Batimastat. Import"],"journal":["ACS chemical neuroscience"],"pubmed_title":["Fluorescent Analogue of Batimastat Enables Imaging of α-Secretase in Living Cells."],"pmcid":["PMC4720558"],"funding_grant_id":["FA9550-12-1-0435","R01 AG006173","P01 AG015379","T32 AG000222-19","AG015379","T32 AG000222","AG000222-19"],"pubmed_authors":["Kim S","Yang J","Selkoe DJ","Leriche G","Chen AC"],"additional_accession":[]},"is_claimable":false,"name":"Fluorescent Analogue of Batimastat Enables Imaging of α-Secretase in Living Cells.","description":"The ADAM family of metalloproteases cleave a diverse range of transmembrane substrates, resulting in the release of their soluble ectodomains. This process of protein shedding, termed α-secretase processing, is involved in many facets of both normal and disease related cellular function. While the processing of substrates has been well documented, the regulation and trafficking of the ADAMs are less well understood. Tools that allow for the study of ADAMs under their native environment will allow for a better understanding of their regulation and activity. Here we describe the design and evaluation of a novel fluorescent analogue of a well-characterized ADAM inhibitor, Batimastat. This probe exhibited similar activity for inhibiting α-secretase processing in cells as did Batimastat. Import","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Jan","modification":"2025-04-22T20:46:03.105Z","creation":"2019-03-27T02:07:15Z"},"accession":"S-EPMC4720558","cross_references":{"pubmed":["26559179"],"doi":["10.1021/acschemneuro.5b00283"]}}