{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(2)"],"submitter":["Lei KE"],"pubmed_abstract":["Ectodysplasin (<i>EDA</i>) gene mutation is associated with hypohidrotic ectodermal dysplasia (HED). The aim of this study was to investigate the effect of ectodysplasin, transcript variant 1 (EDA-A1) on the proliferation and cell cycle of ECV304 human umbilical vein endothelial cells (HUVECs). Recombinant eukaryotic expression vectors containing mutant (M) and wild-type (W) <i>EDA-A1</i> coding sequences, pcDNA3.1 (-)-EDA-A1-M and pcDNA3.1 (-)-EDA-A1-W, respectively, were transfected into ECV304 cells. The EDA-A1 gene was amplified by reverse transcription polymerase chain reaction (RT-PCR), and the protein was detected by western blotting. The EDA-A1 gene and protein were detected in ECV304 cells transfected with pcDNA3.1 (-)-EDA-A1-M and pcDNA3.1 (-)-EDA-A1-W, but not in ECV304 cells tr"],"journal":["Experimental and therapeutic medicine"],"pagination":["535-539"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4734069"],"repository":["biostudies-literature"],"pubmed_title":["Effect of an <i>EDA-A1</i> gene mutant on the proliferation and cell cycle distribution of cultured human umbilical vein endothelial cells."],"pmcid":["PMC4734069"],"pubmed_authors":["Ma B","Che T","Lei KE","Shi P","He X","Li L","Wang L"],"additional_accession":[]},"is_claimable":false,"name":"Effect of an <i>EDA-A1</i> gene mutant on the proliferation and cell cycle distribution of cultured human umbilical vein endothelial cells.","description":"Ectodysplasin (<i>EDA</i>) gene mutation is associated with hypohidrotic ectodermal dysplasia (HED). The aim of this study was to investigate the effect of ectodysplasin, transcript variant 1 (EDA-A1) on the proliferation and cell cycle of ECV304 human umbilical vein endothelial cells (HUVECs). Recombinant eukaryotic expression vectors containing mutant (M) and wild-type (W) <i>EDA-A1</i> coding sequences, pcDNA3.1 (-)-EDA-A1-M and pcDNA3.1 (-)-EDA-A1-W, respectively, were transfected into ECV304 cells. The EDA-A1 gene was amplified by reverse transcription polymerase chain reaction (RT-PCR), and the protein was detected by western blotting. The EDA-A1 gene and protein were detected in ECV304 cells transfected with pcDNA3.1 (-)-EDA-A1-M and pcDNA3.1 (-)-EDA-A1-W, but not in ECV304 cells tr","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Feb","modification":"2025-04-04T20:26:06.562Z","creation":"2019-03-27T02:07:56Z"},"accession":"S-EPMC4734069","cross_references":{"pubmed":["26893642"],"doi":["10.3892/etm.2015.2952"]}}