biostudies-literatureUnknown11(2)Lei KEEctodysplasin (<i>EDA</i>) gene mutation is associated with hypohidrotic ectodermal dysplasia (HED). The aim of this study was to investigate the effect of ectodysplasin, transcript variant 1 (EDA-A1) on the proliferation and cell cycle of ECV304 human umbilical vein endothelial cells (HUVECs). Recombinant eukaryotic expression vectors containing mutant (M) and wild-type (W) <i>EDA-A1</i> coding sequences, pcDNA3.1 (-)-EDA-A1-M and pcDNA3.1 (-)-EDA-A1-W, respectively, were transfected into ECV304 cells. The EDA-A1 gene was amplified by reverse transcription polymerase chain reaction (RT-PCR), and the protein was detected by western blotting. The EDA-A1 gene and protein were detected in ECV304 cells transfected with pcDNA3.1 (-)-EDA-A1-M and pcDNA3.1 (-)-EDA-A1-W, but not in ECV304 cells trExperimental and therapeutic medicine535-539https://www.ebi.ac.uk/biostudies/studies/S-EPMC4734069biostudies-literatureEffect of an <i>EDA-A1</i> gene mutant on the proliferation and cell cycle distribution of cultured human umbilical vein endothelial cells.PMC4734069Ma BChe TLei KEShi PHe XLi LWang LfalseEffect of an <i>EDA-A1</i> gene mutant on the proliferation and cell cycle distribution of cultured human umbilical vein endothelial cells.Ectodysplasin (<i>EDA</i>) gene mutation is associated with hypohidrotic ectodermal dysplasia (HED). The aim of this study was to investigate the effect of ectodysplasin, transcript variant 1 (EDA-A1) on the proliferation and cell cycle of ECV304 human umbilical vein endothelial cells (HUVECs). Recombinant eukaryotic expression vectors containing mutant (M) and wild-type (W) <i>EDA-A1</i> coding sequences, pcDNA3.1 (-)-EDA-A1-M and pcDNA3.1 (-)-EDA-A1-W, respectively, were transfected into ECV304 cells. The EDA-A1 gene was amplified by reverse transcription polymerase chain reaction (RT-PCR), and the protein was detected by western blotting. The EDA-A1 gene and protein were detected in ECV304 cells transfected with pcDNA3.1 (-)-EDA-A1-M and pcDNA3.1 (-)-EDA-A1-W, but not in ECV304 cells tr2016-01-01T00:00:00Z2016 Feb2025-04-04T20:26:06.562Z2019-03-27T02:07:56ZS-EPMC47340692689364210.3892/etm.2015.2952